Title:
MICROPHYSIOLOGICAL SYSTEM
Document Type and Number:
WIPO Patent Application WO/2024/096090
Kind Code:
A1
Abstract:
Disclosed is a microphysiological system comprising a microfluidic device, and human renal proximal tubular epithelial cells (RPTEC) and pluripotent stem cell-derived LTL-positive cells which are accommodated in the microfluidic device. The microfluidic device comprises a device body, a first chamber provided to the device body, a second chamber provided to the device body, and a porous membrane which is positioned between the first chamber and the second chamber, and separates the first chamber and the second chamber. The RPTEC and the LTL-positive cells are accommodated inside the first chamber and are attached to a first surface of the porous membrane facing the first chamber.
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Inventors:
YOKOKAWA RYUJI (JP)
RAMIN BANAN SADEGHIAN (JP)
UENO RYOUHEI (JP)
ARAOKA TOSHIKAZU (JP)
TAKASATO MINORU (JP)
SAHARA YOSHIKI (JP)
RAMIN BANAN SADEGHIAN (JP)
UENO RYOUHEI (JP)
ARAOKA TOSHIKAZU (JP)
TAKASATO MINORU (JP)
SAHARA YOSHIKI (JP)
Application Number:
PCT/JP2023/039543
Publication Date:
May 10, 2024
Filing Date:
November 02, 2023
Export Citation:
Assignee:
RIKEN (JP)
International Classes:
C12M3/00; C12M1/00; C12N5/071; C12N5/10; G01N33/15; G01N37/00
Domestic Patent References:
| WO2018216743A1 | 2018-11-29 | |||
| WO2018070346A1 | 2018-04-19 |
Other References:
RAMIN BANAN SADEGHIAN: "Cells sorted off hiPSC-derived kidney organoids coupled with immortalized cells reliably model the proximal tubule", COMMUNICATIONS BIOLOGY, NATURE PUBLISHING GROUP UK, vol. 6, no. 1, XP093166526, ISSN: 2399-3642, DOI: 10.1038/s42003-023-04862-7
KAWAKAMI, AKIHIKO ET AL.: "Evaluation of membrane transporters in MPS using human iPS cell-derived proximal tubular cells", PROCEEDINGS OF SYMPOSIUM ON "SENSORS/MICROMACHINES AND APPLICATION SYSTEMS"; SENSORS AND MICROMACHINES DIVISION, IEE JAPAN [ED.], 7 November 2022 (2022-11-07), pages 1/5 - 5/5
LIN NEIL Y. C., HOMAN KIMBERLY A., ROBINSON SANLIN S., KOLESKY DAVID B., DUARTE NATHAN, MOISAN ANNIE, LEWIS JENNIFER A.: "Renal reabsorption in 3D vascularized proximal tubule models", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, vol. 116, no. 12, 19 March 2019 (2019-03-19), pages 5399 - 5404, XP055971720, ISSN: 0027-8424, DOI: 10.1073/pnas.1815208116
MINORU TAKASATO, PEI X ER, HAN S CHIU, MELISSA H LITTLE: "Generation of kidney organoids from human pluripotent stem cells", NATURE PROTOCOLS, NATURE PUBLISHING GROUP, GB, vol. 11, no. 9, 1 September 2016 (2016-09-01), GB , pages 1681 - 1692, XP055679831, ISSN: 1754-2189, DOI: 10.1038/nprot.2016.098
TSUJIMOTO, HIKARU, ET AL.: "Generation of Functional Human Kidney Tissues from Metanephric Nephron Progenitors and Ureteric Bud Cells Separately Differentiated from Human iPS Cells", JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, vol. 30, 2019, pages 53 FR-OR 047
TIANZI ZHANG: "Open microfluidic coculture reveals paracrine signaling from human kidney epithelial cells promotes kidney specificity of endothelial cells", AMERICAN JOURNAL OF PHYSIOLOGY: RENAL PHYSIOLOGY, AMERICAN PHYSIOLOGICAL SOCIETY, UNITED STATES, vol. 319, no. 1, 1 July 2020 (2020-07-01), United States , pages F41 - F51, XP093166531, ISSN: 1931-857X, DOI: 10.1152/ajprenal.00069.2020
KAWAKAMI, AKIHIKO ET AL.: "Evaluation of P-gp and SGLT2 transport capacity in MPS using human iPS cell-derived proximal tubular cells", THE PROCEEDINGS OF MECHANICAL ENGINEERING CONGRESS, JAPAN, vol. 2022, 5 September 2022 (2022-09-05), pages J025-06
KAWAKAMI, AKIHIKO ET AL.: "Evaluation of membrane transporters in MPS using human iPS cell-derived proximal tubular cells", PROCEEDINGS OF SYMPOSIUM ON "SENSORS/MICROMACHINES AND APPLICATION SYSTEMS"; SENSORS AND MICROMACHINES DIVISION, IEE JAPAN [ED.], 7 November 2022 (2022-11-07), pages 1/5 - 5/5
LIN NEIL Y. C., HOMAN KIMBERLY A., ROBINSON SANLIN S., KOLESKY DAVID B., DUARTE NATHAN, MOISAN ANNIE, LEWIS JENNIFER A.: "Renal reabsorption in 3D vascularized proximal tubule models", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, NATIONAL ACADEMY OF SCIENCES, vol. 116, no. 12, 19 March 2019 (2019-03-19), pages 5399 - 5404, XP055971720, ISSN: 0027-8424, DOI: 10.1073/pnas.1815208116
MINORU TAKASATO, PEI X ER, HAN S CHIU, MELISSA H LITTLE: "Generation of kidney organoids from human pluripotent stem cells", NATURE PROTOCOLS, NATURE PUBLISHING GROUP, GB, vol. 11, no. 9, 1 September 2016 (2016-09-01), GB , pages 1681 - 1692, XP055679831, ISSN: 1754-2189, DOI: 10.1038/nprot.2016.098
TSUJIMOTO, HIKARU, ET AL.: "Generation of Functional Human Kidney Tissues from Metanephric Nephron Progenitors and Ureteric Bud Cells Separately Differentiated from Human iPS Cells", JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, vol. 30, 2019, pages 53 FR-OR 047
TIANZI ZHANG: "Open microfluidic coculture reveals paracrine signaling from human kidney epithelial cells promotes kidney specificity of endothelial cells", AMERICAN JOURNAL OF PHYSIOLOGY: RENAL PHYSIOLOGY, AMERICAN PHYSIOLOGICAL SOCIETY, UNITED STATES, vol. 319, no. 1, 1 July 2020 (2020-07-01), United States , pages F41 - F51, XP093166531, ISSN: 1931-857X, DOI: 10.1152/ajprenal.00069.2020
KAWAKAMI, AKIHIKO ET AL.: "Evaluation of P-gp and SGLT2 transport capacity in MPS using human iPS cell-derived proximal tubular cells", THE PROCEEDINGS OF MECHANICAL ENGINEERING CONGRESS, JAPAN, vol. 2022, 5 September 2022 (2022-09-05), pages J025-06
Attorney, Agent or Firm:
HASEGAWA Yoshiki et al. (JP)
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