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WO/1993/020236A1 |
Method and composition for distinguishing different-sequence polynucleotides electrophoretically in a non-sieving medium. In practicing the method, there are formed one or more different-sequence polynucleotide(s) which contain (i) a det...
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WO/1993/020434A1 |
A method for electrophoresis of nucleic acid fragments present in the solution which contains an amount, e.g., 0.2 % or more, of a reagent, e.g., glycerol, dithiolthreitol (DTT) and trehalose or other sugars, which interact to form a com...
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WO/1993/020239A1 |
Method and composition for detecting one or more selected polynucleotide regions in a target polynucleotide. In practicing the method, a plurality of different-sequence probe pairs are added to a target polynucleotide, where each probe p...
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WO/1993/016789A1 |
A liquid stream electrophoresis apparatus including a body (12, 14, 16, 18, 32, 38) defining a substantially parallelepiped space, and a first set of electrodes (24, 26) placed on the smaller sides as defined lengthwise and widthwise. Sa...
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WO/1993/016788A1 |
In a process for electroelution of a gel containing separated charged macromolecules, such as proteins or DNA/RNA, a preparatory separation after pre-equilibration of the gel is performed by collective elution of the entire gel in an ele...
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WO/1993/015394A1 |
Disclosed herein are capillary columns for capillary electrophoretic analysis of sample constituents. In an embodiment, the columns comprise a bifunctional layer comprising a positively charged amine group adsorbed to the inner wall of t...
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WO/1993/015392A1 |
An electrophoresis-based assay system for detection of one or more target substances, i.e. an analyte tagged with fluorescent binding agents. The analyte is reacted with an excess amount of fluorescently tagged binding agent. The reactio...
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WO/1993/010442A1 |
An apparatus and method of continuous flow zone electrophoresis for separation of particulate or biological samples, including cells and sub-cellular components, in a separation chamber configured to enhance separation of the sample comp...
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WO/1993/008903A1 |
A process is disclosed for separating enantiomers by an electrokinetic process in capillary columns, using surface-immobilized chiral components. The chiral component used may be a polysiloxane to which alkylated cyclodextrine (alpha, be...
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WO/1993/006475A1 |
Improved capillary electrophoresis apparatus of the type that generally includes a capillary tube, the capillary tube having a sample inlet end and an outlet end, a first electrode in electrical communication with the inlet end of the ca...
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WO/1993/005389A1 |
Disclosed herein is a dynamic coating buffer useful in the analysis of samples by open-tube capillary electrophoretic methods using untreated fused silica capillary columns. The dynamic coating buffer comprises at least one agent having ...
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WO/1993/003815A1 |
The present invention relates to the direct electrolysis of tall oil soap (TOS) to produce crude tall oil (CTO). An aqueous tall oil soap solution (22) generated in a kraft wood-pulping process is placed in contact with an anode (34) in ...
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WO/1993/000986A1 |
A method and apparatus for electrophoretic gel imaging in which two transparent plates are secured together to define between them at least one track for electrophoretic gel which has a thickness or depth in the range 25 to 250 microns, ...
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WO/1993/000583A1 |
An electrophoresis device having a single-piece cartridge (10) filled with a suitable and optionally dyed gel (26) and made from a section of a hollow shape having two opposite open sides (20,22), one of which is sealed by a removable co...
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WO/1992/020434A1 |
The invention concerns an electrophoresis chamber constructed from a multiplicity of cooled elements.
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WO/1992/019686A2 |
A process and apparatus for treating overspray from the spray application of waterborne coatings is provided, which includes at least an electrophoresis separation step (means) (26) for recovering substantially all of solids and higher m...
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WO/1992/014538A1 |
A method of manufacturing a gel slab for the separation and transfer of macromolecules by electrophoresis involves securing the edge of a microporous membrane (10) to a frame (12) and pouring the liquid gel into the frame while avoiding ...
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WO/1992/012789A1 |
An agarose electrophoretic gel, a method for forming the gel, and a solid intermediate gamma-irradiated clarified galactomannan (gammaCGM) for use in the gel. The gel is formed by the addition of gammaCGM to optically clarify the gel for...
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WO/1992/011531A1 |
The present invention provides for the use of the fluorescent label 2-aminoacridone for use in separating carbohydrate mixtures and analyzing the structure of carbohydrates. Carbohydrates for analysis may be labelled by 2-aminoacridone a...
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WO/1992/011077A1 |
Ampholytic solute zones which have been stabilized by isoelectrical focusing in a separation medium such as a capillary are mobilized by the introduction of a relatively large quantity of zwitterion to one of the electrode chambers. When...
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WO/1992/011078A1 |
Spontaneous and undesired electroendosmosis in a capillary during an electrophoretic separation procedure is suppressed by the inclusion of a viscosity-raising additive in one or both of the electrode chamber solutions. The additive is e...
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WO/1992/010272A1 |
The frame is comprised of two small bars (1, 2) braced by two pairs of elastic blades (3,4) and (5, 6). Cams (11, 12) bias said blades to bring the small bars (1, 2) closer to each other. The membrane is then fixed under the bars (1, 2) ...
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WO/1992/010277A1 |
This invention relates to the formation and use of a variable, multilayer system of discrete, discontinuous and nonsequential molecular sieves, comprising of agarose-acrylic gels of distinct composition. Each of the individual gel-layers...
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WO/1992/006773A1 |
A method and compositions for separating molecules based on molecular size, shape, affinity, chirality, weight, charge, and hydrogen bonding, using a mosaic matrix formed by polymerizing a dispersion of dispersoids within a polymeric mat...
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WO/1992/004625A1 |
The invention relates to a process and homogenous electrophoresis plate for protein separation by SDS electrophoresis using a novel tris-formiate-taurinate buffer system.
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WO/1992/000795A1 |
An electrophoresis chamber of modular design with capillary cooling system non-dependent on chamber size.
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WO/1991/017815A1 |
The present invention includes novel apparatus and methods for isoelectric focusing of amphoteric substances within fluids containing carrier ampholytes. The present invention utilizes multichannel recycling isoelectric focusing techniqu...
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WO/1991/016966A1 |
A microfluidic structure comprises first and second substantially planar form-stable base layers (1, 2), and an intermediate spacing layer (3) of elastic material, said spacing layer (3) being recessed to define a microcavity or channel ...
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WO/1991/014489A1 |
Electrophoretic media based on polymers with novel structures are disclosed. The polymers are formed by polymerization of acrylamide monomers, such as N-methylolacrylamide, with cross-linking agents or comonomer agents such as tetraethyl...
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WO/1991/012904A1 |
Devices and methods are disclosed for moving charged molecules through a medium by the application of a plurality of electrical fields of sufficient strength and applied for sufficient amounts of time so as to move the charged molecules ...
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WO/1991/012073A1 |
Capillary zone electrophoresis is enhanced by the application of an electric field across the interior of the capillary tube (10). This external electric field is applied through a conductive member (104) at the exterior of the capillary...
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WO/1991/012071A1 |
A method for separating solid matter from a suspension thereof in a liquid carrier by using electrophoretic deposition. The method involves, in an electrophoretic deposition cell, passing an electric current through said suspension betwe...
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WO/1991/011709A1 |
A supported, substantially uniform matrix for use in electric field-induced separation of molecular components in a sample. The matrix is prepared by forming a viscoelastic polymer matrix and pumping the matrix into a capillary or prepar...
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WO/1991/011262A1 |
By applying two or more electrical fields (DC, AC, pulsed) of different characteristics to an electrode array on e.g. the wall of a treatment cell, particles suspended in liquid in the cell may be manipulated as desired on a microscopic ...
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WO/1991/010129A1 |
A protein purification system based upon isoelectric focusing and isotachophoresis includes a separation chamber (100) having matched sets of inlet and outlet ports. The outlet ports are connected to the inlet ports by tubing modules (13...
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WO/1991/006851A1 |
A method for achieving desired electroosmotic flow characteristics in a capillary tube (22) having charged surface groups. An electrolyte solution (28) containing a compound effective to stably alter the charge of the tube walls is drawn...
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WO/1991/005598A1 |
Fabric supported agarose gels with a high polyol content are useful in electrophoresis.
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WO/1991/005265A1 |
Charged carbohydrate substances which have been run on an electrophoretic gel are transferred onto a porous membrane by a blotting technique such as an electrotransfer technique. This enables recovery of carbohydrate substances from a ge...
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WO/1991/004095A1 |
The present invention produces very stable, covalently bonded separation substrates for separations application such as liquid and gas chromatography as well as capillary zone electrophoresis. An intermediate substrate is prepared which ...
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WO/1991/004085A1 |
Method and apparatus for conducting electrophoretic separations. The invention permits adequate heat removal during separation so that the process can be conducted efficiently on a batch or continuous basis. The invention further provide...
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WO/1991/002577A1 |
A blotting composition is provided, comprising a transfer substrate such as a gel containing, e.g., DNA, RNA or a protein, applied to a polyaldehyde activated microporous polymer membrane having A) an internal surface which includes pore...
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WO/1991/002576A1 |
A technique for passaging liquid through a membrane putatively containing, in its interstices, at least one substance for which detection is desired, comprises positioning donor and acceptor bibulous matrices onto either surface of the m...
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WO/1991/002815A1 |
In a process for separating and detecting constituents of a mixture of substances by temperature gradient gel electrophoresis (TGGE), a spatial temperature gradient is constructed from spatially separate temperature levels, or a temporal...
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WO/1991/001995A1 |
Purified agarose suitable for rapid electrophoresis, characterized by a sulfate content of less than 0.2 wt. % but greater than zero, a pyruvate content of 0-0.1 wt. %, and a nitrogen content of 0-0.02 wt. %. Gels prepared from the agaro...
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WO/1991/002244A1 |
A method of fractionating nucleic acid fragments is disclosed. The method is based on counter migration of the fragments in an upstream direction through a polymer solution which is moving by electroosmotic flow in a downstream direction...
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WO/1990/015987A1 |
Bulk fluid movement in a capillary electrophoresis system is achieved by imposing a temperature change on either a portion of the capillary or a bulb joined to the capillary at one end. The process may be used for sample loading, in whic...
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WO/1990/013813A1 |
A method and apparatus are presented for the casting of gradient gels, without voids, in confined support structures such as thin slab-type configurations. Furthermore, by using a differential activation scheme for the casting, the metho...
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WO/1990/008807A1 |
This invention relates to porous substrates with a high concentration of amines which are useful for transfer of substances from a gel or culture.
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WO/1990/007978A1 |
Electrophoretic resolving gel compositions, discontinuous stacking gel systems containing these or other resolving gel compositions, and kits for preparing such gel systems, comprising one or more polysaccharide hydrogels wherein at leas...
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WO/1990/005017A1 |
A pulsed field electrophoresis method and apparatus for the separation of molecules wherein a fixed polarity potential difference between an anode and a cathode, disposed at respective opposed edges of the gel, produces a fixed polarity ...
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