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WO/2002/062984A1 |
The invention involves a method of simulating bioelectricity to activate the recessive gene of normal yeast to convert it a specific yeast. The specific proteins (enzymes) expressed by the specific yeast have the ability to activate, mod...
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WO/2002/062982A1 |
The invention relate to a method adoptting simulative bio-electric-wave information, which activates recessive gene of normal yeast, and converts normal yeast to specific yeast, the specific protein(enzyme) expressed by specific yeast re...
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WO/2002/059277A2 |
Compositions, methods and systems are provided for the stimulation of biological activities within stem cells by applying electromagnetic stimulation to an electroactive material. In general the present invention involves attaching or as...
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WO/2002/033066A1 |
Disclosed is a method for penetration of lipid bilayer membranes in order to insert at the tip of a hollow needle-shaped object, such as a micropipet-, into a container formed of a lipid bilayer membrane, wherein said container is placed...
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WO/2002/033065A2 |
The invention concerns a method for producing proteins which consists in subjecting a liquid medium flow comprising at least a yeast, bacterium or cell of mammals to electroporation, and recuperating the released proteins. The method com...
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WO/2002/030562A1 |
The invention includes a composition that is an integrated biochip system for processing and analyzing samples using sequential tasks that take place on one or more chips. The system preferably comprises one or more active chips, and can...
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WO/2002/029076A1 |
Chemical reactions are accelerated by applying an electromagnetic field to result in dielectric heating of a solid material containing a bound retractant. A composite is provided containing a solid dielectric material susceptibl to diele...
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WO/2002/020431A1 |
The present invention provides biological fertilizer compositions that comprise yeast cells that have an enhanced ability to fix atmospheric nitrogen, decompose phosphorous minerals and compounds, decompose potassium minerals and compoun...
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WO/2002/006440A1 |
A new and useful apparatus for producing cell electrofusion is provided. The apparatus comprises: a. a chamber with a substrate disposed therein; b. means for directing the cells to be fused toward one side of the substrate; and c. a dev...
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WO/2002/000871A2 |
The invention relates to a novel method which permits the transport of DNA and/or other biologically active molecules into the nucleus of higher eukaryotic cells by means of an electrical current, independent of the cell division and wit...
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WO/2001/081608A2 |
Recombinant expression vectors and methods are provided for detecting HIV and monitoring HIV drug resistance. The method comprises: taking a culture of cells; adding a recombinant viral vector into the culture to transduce the cells, the...
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WO/2001/068110A1 |
A method and apparatus are provided to induce a response in cells by illuminating cells to determine which are labeled with a first label with a first wavelength of light and which are labeled with other labels with other wavelengths of ...
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WO/2001/066694A2 |
The invention relates to a method for transferring material through the membrane of at least one cell, whereby the transfer is carried out in the presence of trehalose. This method can be particularly utilized in the fields of genetic en...
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WO/2001/062336A1 |
Methods and devices (10) for the regulation of gene expression by cells via the application of specific and selective electric and electromagnetic signals so as to target diseased or injured tissue for treatment. Gene expression is the u...
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WO/2001/060994A1 |
The present invention provides a method and apparatus for selectively destroying dividing cells in living tissue formed of dividing cells and non-dividing cells. The dividing cells contain polarizable intracellular members and during lat...
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WO/2001/058431A1 |
We describe a method of producing a red blood cell suitable for delivery of an agent to a vertebrate, the method comprising: (a) providing a red blood cell; (b) pre-sensitising the red blood cell; and (c) loading the red blood cell with ...
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WO/2001/058432A1 |
We describe an apparatus for providing a red blood cell suitable for delivery of an agent to a vertebrate, the apparatus comprising: (a) a sensitisation means for sensitising a red blood cell to render it susceptible to disruption by an ...
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WO/2001/059085A1 |
Methods for protection of a biomolecule of interest in a biologically derived composition to be subjected to illumination with at least one high-intensity, short duration pulse of incoherent polychromatic light in a broad spectrum (BSPL)...
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WO/2001/056938A1 |
Anaerobically decomposed organic materials such as landfill material or sewage sludge are treated by passage of an electric current therethrough in order to enhance production of hydrogen, suppress formation of methane and decrease the t...
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WO/2001/051625A2 |
Apparatus and method for enhanced biomass production by electrical waveform shaping are disclosed. The invention relates to cultivating a biological source cell in a liquid-medium bioreactor and applying a waveform regulated electric fie...
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WO/2001/040453A1 |
A process and system for electrical extraction of intracellular matter from biological matter, and intracellular matter products formed thereby, based on preparing a mixture of biological matter featuring cells, and an electro-conductive...
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WO/2001/040454A1 |
This invention provides a method and apparatus for selectively identifying, and targeting with an energy beam, specific cells within a cell population, for the purpose of inducing a response in the targeted cells. Using the present inven...
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WO/2001/030172A1 |
A process for producing cheese, cheese analogues and cheese-derived products comprises contacting said cheese, analogues or products with a Kluyveromyces lactis cell or an enzyme derived therefrom.
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WO/2001/019963A2 |
Efficient cell lysis in small samples, i.e., samples less than one milliliter, is achieved by exposing the sample to microwave radiation in the frequency range of 18 to 26 GHz. The sample containing cells is supported in a wave-guide cav...
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WO/2001/017687A2 |
A method is provided for modifying cells retained in a high gradient magnetic cell separation column (HGMS). Selected cells within a heterogeneous mixture are labeled with a label comprising a magnetic particle and specific for the selec...
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WO/2001/014870A1 |
Methods and apparatus for discriminating matter in a chamber having an inlet port and an outlet port utilizing dielectrophoresis and field flow fractionation. A carrier medium is introduced into the inlet port and is directed from the in...
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WO/2001/010319A1 |
A method for intracellular electro-manipulation is provided. The method includes applying one or more ultrashort electric field pulse to target cells. The ultrashort electric field pulses have sufficient amplitude and duration to modify ...
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WO/2001/009297A1 |
Disclosed is a method for selective electrofusion of at least two fusion partners having cell-like membranes and cellular or subcellular dimensions, comprising the following steps: A) the fusion partners are brought into contact with eac...
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WO/2001/009362A1 |
This invention provides methods for producing and selecting host cells that better survive transformation treatment by subjecting host cells to conditions that alter them, subjecting the altered cells to transformation conditions, and se...
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WO/2001/007585A1 |
Images created by electrical impedance tomography (EIT) are used to adjust one or more electrical parameters and obtain a desired degree of electroporation of cells in tissue. The parameters include current, voltage and a combination the...
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WO/2001/007910A1 |
The activity of intracellular chemical reactions of molecules is measured by the use of fluorescently labeled substrate molecules (114) that undergo a change in electrophoretic mobility upon chemical reaction such as that catalyzed by an...
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WO/2001/007584A1 |
Electroporation is performed in a controlled manner in either individual or multiple biological cells or biological tissue by monitoring the electrical impedance, defined herein as the ratio of current of voltage in the electroporation c...
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WO/2001/007583A1 |
An electrical current is created across an electrically conductive medium comprising a cell which may be part of a tissue of a living organism. A first electrical parameter which may be current, voltage, or electrical impedance is measur...
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WO/2001/005214A1 |
A device for the manipulation of a biological material by a magnetic field is presented. The device comprises a magnetic field source (10) coupled to a current source. The current source is of a kind supplying an electric current of at l...
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WO/2000/077186A2 |
A bacterial cell having protection against conditions which are lethal to an unprotected bacterial cell wherein, the protected cell is obtained by subjecting a bacterial cell to treatment with a sublethal level of stress.
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WO/2000/063355A1 |
The invention relates to a method for treating an aqueous flux which is colonized by cells by means of a pulsed electric field which is applied to the flux, characterized in that the applied field is substantially parallel to the flow of...
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WO/2000/060065A1 |
An object of the invention is to provide a tangential flow, cell concentration and fusion apparatus (12) which includes a transparent housing (24) and a first input channel (1) contained in the housing (24). In the housing (24), a concen...
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WO/2000/060072A1 |
A method for polynucleotide synthesis, comprises the steps of: (i) reacting a polynucleotide processive enzyme, e.g. a polymerase or TdT, with a nucleotide substrate under conditions suitable for enzyme activity; and (ii) modulating the ...
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WO2000017322A9 |
Compositions, methods and systems are provided for the stimulation of biological activities within cells by applying electromagnetic stimulation to an electroactive material, wherein the electromagnetic stimulation is coupled to the elec...
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WO/2000/060049A1 |
The invention concerns an apparatus comprising a sonotrode (2) designed to generate more or less high power ultrasounds in at least a biological sample (5) containing cells to be lysed, which sample is contained in at least a receptacle ...
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WO/2000/056870A1 |
Provided is a cell culture apparatus (12) for culturing cells, and optionally, for performing magnetic separation of cells desired to be cultured. The cell culture apparatus (12) preferably comprises a frame (18); two membranes (31, 32),...
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WO/2000/043515A2 |
A method is provided for detecting a presence of HIV virus in a sample comprising: taking a culture of recombinant cells which (a) are capable of cell division; (b) express CD4 receptor and one or more additional cell surface receptors n...
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WO/2000/037628A1 |
According to the invention, electroporation and/or fusion treatment of microscopic objects is effected in a medium between at least two electrodes, wherein said electrodes are miniaturized electrodes in a microsystem with a channel struc...
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WO/2000/034454A2 |
Microcellular polyhipe polymer scaffold suitable for growth of living matter for biomedical applications, obtainable by polymerising a high internal phase emulsion, comprising a homogeneous cross-linked open cellular material defined by ...
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WO/2000/026405A1 |
Bacteria cells and/or spores are collected and concentrated to form a specimen that is lysed using an ionized fluid to facilitate identification of the bacterial cells or spores by tests performed on the DNA and RNA contained therein. An...
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WO/2000/022147A1 |
This invention provides improved electroporation methods for transferring nucleic acids of interest into host cells, wherein the host cells are (1) suspended in a substantially non-ionic solution comprising at least one sugar or sugar de...
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WO/2000/020045A1 |
The present invention relates to a method and apparatus for effective inactivation of micro-organisms in fluids with relatively high absorbance so as to limit damage. The apparatus has a large diameter passage (2) flow-through UV radiati...
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WO1999067427A9 |
This invention relates to antiviral drug susceptibility and resistance tests to be used in identifying effective drug regimens for the treatment of human immunodeficiency virus (HIV) infection and acquired immunodeficiency syndrome (AIDS...
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WO1999064581A9 |
An apparatus is provided for producing cell electrofusion (20) wherein the cells to be fused are directed to and retained on one side of a substrate (34) and between the electrodes (30, 32). Thus, a significant portion of the cells are i...
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WO/2000/003447A1 |
Disclosed are methods using neutral red to mediate the interconversion of chemical and electrical energy. Electrically reduced neutral red has been found to promote cell growth and formation of reduced products by reversibly increasing t...
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