Title:
【発明の名称】細胞内成分を用いる無傷細胞の固相強化
Document Type and Number:
Japanese Patent JP2000504213
Kind Code:
A
Abstract:
The present invention provides a simple, cost-effective method, and kit, for enriching one or more target cells from a mixed cell population. According to the invention, target cells are detected by a detecting agent which attaches to an intracellular constituent of the target cell, for example, a nucleic acid, peptide, protein, etc., in the cytoplasm underlying the outer cell membrane or outer cell wall. The detected cells are then concentrated from the mixed population of cells using a solid phase support system which may include an immunoaffinity or immunomagnetic system. The enriched cells may then be identified and visualized using an identifying agent and a signal generating system. The present invention also provides a method for increasing the sensitivity of the enrichment of cells from a mixed population of cells by amplifying a selected intracellular constituent of the target cell prior to enrichment.
Inventors:
Carionis, building
Application Number:
JP52552997A
Publication Date:
April 11, 2000
Filing Date:
January 17, 1997
Export Citation:
Assignee:
Flinders Technologies Proprietary Limited
International Classes:
G01N33/53; C12N5/00; C12N5/07; C12N5/071; C12N11/02; C12N11/14; C12Q1/68; C12Q1/6804; C12Q1/6841; G01N33/543; (IPC1-7): C12N11/14; C12N5/06; C12N11/02; C12Q1/68; G01N33/53; G01N33/543
Attorney, Agent or Firm:
Aoyama Ryo (1 person outside)