Title:
【発明の名称】核酸配列を含む粒子を酵母中で生成する方法
Document Type and Number:
Japanese Patent JP2000506742
Kind Code:
A
Abstract:
A method of synthesizing particles containing nucleic acid sequences is disclosed. This method comprises the steps of introducing a non-endogenous packageable nucleic acid sequence and a sequence encoding a suitable non-endogenous capsid protein into yeast cells and inducing particle assembly, wherein at least one nucleic acid is encapsidated. Preferably, the particle is an infectious virion and the method additionally comprises the step of inducing viral nucleic acid replication before particle assembly. A method of replicating an animal or Nodaviridae virus nucleic acid sequences in yeast is also disclosed.
Inventors:
Price, Deuan Bee.
Lucato, Roland Earl.
Chlor, Michael A.
Archist, Paul G.
Lucato, Roland Earl.
Chlor, Michael A.
Archist, Paul G.
Application Number:
JP50598398A
Publication Date:
June 06, 2000
Filing Date:
March 21, 1997
Export Citation:
Assignee:
Wisconsin Alumni Research Foundation
International Classes:
C12N15/09; C07K14/08; C12N7/00; C12N15/81; (IPC1-7): C12N15/09; C07K14/08; C12N7/00
Attorney, Agent or Firm:
Takao Kawasaki (1 outside)