Title:
【発明の名称】安定性が2個のハイブリッドされた核酸分子の塩基組成に僅かしか依存しないハイブリッド複合体の形成方法
Document Type and Number:
Japanese Patent JP2000512843
Kind Code:
A
Abstract:
The invention discloses a method for producing a hybridization complex whose stability is substantially independent of the base composition of two hybridized nucleic acid molecules, consisting in contacting a first nucleic acid molecule with a second nucleic acid molecule under conditions enabling the formation of a specific hybridization complex. The invention is characterized in that at least one of the four types of bases contained in the sequence of the first and/or of the second nucleic acid molecule is a modified base having pairing properties close to those of the natural base it replaces, such that at least one of the two types of the base pair contained in the composition of the complex comprises a modified base and in that the totality of the base pairs contained in the composition of the said complex has a like stability.
Inventors:
Enguyen Chuong
Asline Ulysse
Enguyen Hk
Duran morris
Moriso Junk Road
Dupre Daniel
Bonfis Edwig
Asline Ulysse
Enguyen Hk
Duran morris
Moriso Junk Road
Dupre Daniel
Bonfis Edwig
Application Number:
JP50245198A
Publication Date:
October 03, 2000
Filing Date:
June 25, 1997
Export Citation:
Assignee:
Appligen On Call SA
International Classes:
C12N15/09; C07H21/00; C12Q1/68; C12Q1/6827; C12Q1/6832; (IPC1-7): C12Q1/68; C07H21/00
Attorney, Agent or Firm:
Shogo Onishi