Title:
【発明の名称】幹細胞の分化を阻害させるためのデルタ様蛋白質の使用
Document Type and Number:
Japanese Patent JP2000513329
Kind Code:
A
Abstract:
Primitive hematopoietic stem cells are closely associated with discrete in vivo microenvironments. These "niches" are thought to provide the molecular signals that mediate stem cell differentiation and self renewal. We have dissected the fetal liver microenvironment into distinct cellular components by establishing an extensive panel of stromal cell lines. One particular cell line maintains repopulating stem cells for prolonged in vitro culture periods. A subtraction cloning strategy has yielded a cDNA which encodes a cell surface glycoprotein with a restricted pattern of expression among stromal cell lines. This molecule, previously identified as dlk/Pref-1, contains EGF-like repeats which are related to those in the Notch/Delta/Serrate family of proteins. We have investigated the potential role of this molecule in hematopoietic stem/progenitor cell regulation. We show that the dlk protein displays activity on purified stem cells by promoting the formation of "cobblestone areas" of proliferation. These cobblestone areas contain both primitive high-proliferative potential progenitors as well as in vivo repopulating stem cells.
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Inventors:
Witte, Raleigh
Peytosky, Bronislaw
Moor, Kateri, A.
Remiska, Aio, Earl.
Peytosky, Bronislaw
Moor, Kateri, A.
Remiska, Aio, Earl.
Application Number:
JP53121997A
Publication Date:
October 10, 2000
Filing Date:
March 03, 1997
Export Citation:
Assignee:
ImClone Systems, Inc.
Trustees of Princeton University
Trustees of Princeton University
International Classes:
C07K14/705; C12N5/0789; A61K38/00; (IPC1-7): A61K38/00; A61K31/00; A61K31/505; C07K14/47
Attorney, Agent or Firm:
Miyuki Ariga (4 outside)