Title:
【発明の名称】標的核酸配列の増幅方法
Document Type and Number:
Japanese Patent JP2000515382
Kind Code:
A
Abstract:
A target nucleic acid sequence amplification method wherein at least the target nucleic acid sequence, at least one oligonucleotide primer specific for the target sequence, one or more enzymatic activities and nucleotides are provided, and the target sequence is amplified under conditions suitable in particular for said enzymatic or activity or activities, is disclosed. According to the method, at least one of the nucleotides is a prefunctionalized nucleotide that differs from the other nucleotides at least in that it includes at least one unprotected reactive covalence function in at least one predetermined site of the base of said nucleotide, in order to give a prefunctionalized amplificati product including at least one such prefunctionalized nucleotide. Nucleotides for carrying out the method are also disclosed.
Inventors:
Guillo-Bonich, Francoise
Eric Dufranc
Juan, Antoine
Rayun, Ali
Loam jean
Trevisior, Emmanuel
Eric Dufranc
Juan, Antoine
Rayun, Ali
Loam jean
Trevisior, Emmanuel
Application Number:
JP50768198A
Publication Date:
November 21, 2000
Filing Date:
August 01, 1997
Export Citation:
Assignee:
BAEO MERYU
International Classes:
C12N15/09; C07H19/06; C07H21/00; C07H21/02; C07H21/04; C12N15/10; C12P19/30; C12P19/34; C12Q1/68; C12Q1/686; (IPC1-7): C12N15/09; C12Q1/68
Attorney, Agent or Firm:
Masatake Shiga (1 person outside)