Title:
【発明の名称】Ku欠損細胞と非ヒトトランスジェニック動物
Document Type and Number:
Japanese Patent JP2000515386
Kind Code:
A
Abstract:
Ku deficient cells and transgenic animals are described that comprise at least one allele of the XRCC5 gene that has been mutated by targeted disruption. Fibroblasts derived from XRCC5 mutant embryos and mice were found to prematurely age. These cells displayed decreased growth, slow entry into S phase, altered colony size distribution that favored small colonies, short life span and morphology characteristic of terminal differentiation. Mutant cells were also hypersensitive to gamma -radiation. The tissue culture data was at least partly reproduced in vivo because mutant mice grew slower than control littermates. The XRCC5 mutation, designated xrcc5M1, was a deletion of nucleotides 701-964 that shifted the reading frame. xrcc5M1 is expected to be null because the deleted allele produced no detectable transcript and because lymphocyte development and V(D)J recombination was severely disrupted.
Inventors:
Hasty, Paul
Rim, day chic
Rim, day chic
Application Number:
JP50824698A
Publication Date:
November 21, 2000
Filing Date:
August 07, 1997
Export Citation:
Assignee:
M. D. Anderson Cancer Center
International Classes:
A01K67/027; C12N5/10; C12N9/12; C12N15/09; C12N15/85; (IPC1-7): C12N15/09; A01K67/027; C12N5/10
Attorney, Agent or Firm:
Yusuke Hiraki (2 outside)