Title:
【発明の名称】染料二量体化に基づく蛍光発生プロテアーゼ基質
Document Type and Number:
Japanese Patent JP2002506343
Kind Code:
A
Abstract:
A method of biological assay comprises the steps of providing an enzyme substrate comprising two fluorescence dye groups bound to a flexible peptide, the dye groups being of proximity sufficiently close so as to allow free energy attractions to draw the dyes together to essentially self-quench fluorescence of the dye groups, wherein self quenching of fluorescence of the dye groups is effected by dye dimerization or stacking, and enzymatically cleaving the peptide to release the fluorescence dye groups from dye dimerization or stacking, thereby producing an increase in fluorescence intensity. A protease substrate for use in the method of the invention is also disclosed. This invention finds use in detection and identification of microorganisms, sterilization assurance, pharmaceutical discovery, enzyme assays, immunoassays, and other biological assays.
Inventors:
Way, eye-pin
Williams, Michael G.
Williams, Michael G.
Application Number:
JP54802298A
Publication Date:
February 26, 2002
Filing Date:
September 08, 1997
Export Citation:
Assignee:
Minnesota Mining and Manufacturing Company
International Classes:
C07K7/06; C12Q1/04; C12Q1/37; (IPC1-7): C12Q1/37; C07K7/06; C12Q1/04
Attorney, Agent or Firm:
Takashi Ishida (4 others)