Title:
【発明の名称】広範な特異性のDNA損傷エンドヌクレアーゼ
Document Type and Number:
Japanese Patent JP2002517214
Kind Code:
A
Abstract:
The present disclosure describes DNA damage endonucleases which exhibit broad specificity with respect to the types of structural aberrations in double stranded DNA. These enzymes recognize double stranded DNA with distortions in structure, wherein the distortions result from photoproducts, alkylation, intercalation, abasic sites, mismatched base pairs, cisplatin adducts and inappropriately incorporated bases (for example, 8-oxoguanine, inosine, xanthine, among others). The UVDE (Uve1p) of Schizosaccharomyces pombe, certain truncated forms of that UVDE (lacking from about 100 to about 250 amino acids of N-terminal sequence) and certain endonucleases from Homo sapiens, Neurospora crassa, Bacillus subtilis, and from Deinococcus radiodurans. The present disclosure further provides methods for cleaving double stranded DNA having structural distortions as set forth herein using the exemplified endonucleases or their stable, functional truncated derivatives.
More Like This:
Inventors:
Dorch, Paul W.
Cole, Barbine
Avery, Angela Em.
Cole, Barbine
Avery, Angela Em.
Application Number:
JP2000552912A
Publication Date:
June 18, 2002
Filing Date:
June 08, 1999
Export Citation:
Assignee:
Emory University
International Classes:
A61K9/00; A61K38/46; A61P17/00; A61P35/00; A61P43/00; C12N9/22; C12N15/09; A61K38/00; (IPC1-7): C12N15/09; A61K9/00; A61K38/46; A61P43/00; C12N9/22
Attorney, Agent or Firm:
Shusaku Yamamoto