Title:
【発明の名称】異種遺伝子の運搬用の標的化アデノウイルスベクター
Document Type and Number:
Japanese Patent JP2002523105
Kind Code:
A
Abstract:
Modification of internal sites of the adenovirus fiber protein and hexon protein permit effective targeting of adenovirus vectors. Accessible sites to redirect adenovirus targeting were identified. The HVR5 loop of the hexon protein and the HI loop of the fiber protein (knob) were highly permissive for the insertion of foreign protein sequences, which apparently did not impact on the viability and productivity of corresponding viruses. Accessibility and functionality of the epitope strongly depend on the size of the neighboring spacers. Other results suggest that short targeting peptides can be effectively fused to the C-terminus of the fiber protein. In a specific embodiment, a series of adenovirus vectors modified at the HVR5 site, the fiber protein HI loop, or the fiber protein C-terminus to target urokinase-type plasminogen activator receptor bearing cells were prepared. Such vectors are particularly useful for targeting the vasculature, e.g., for gene therapy of cancers or cardiovascular conditions.
Inventors:
Beanille, Emmanuel
Daudeuil, Jean-Francois
Ratta, Martine
Yeah, Patrice
Pelicode, Michel
Daudeuil, Jean-Francois
Ratta, Martine
Yeah, Patrice
Pelicode, Michel
Application Number:
JP2000567724A
Publication Date:
July 30, 2002
Filing Date:
August 27, 1999
Export Citation:
Assignee:
Avantes Furuma S.A.
International Classes:
A61K35/76; A61K45/00; A61K48/00; A61P9/10; A61P11/06; A61P21/04; A61P25/00; A61P31/04; A61P31/18; A61P35/00; A61P37/00; C07K14/075; C07K14/105; C07K14/50; C07K14/78; C12N7/00; C12N7/01; C12N15/09; C12N9/72; C12N15/34; C12N15/861; C12R1/92; (IPC1-7): C12N15/09; A61K35/76; A61K45/00; A61K48/00; A61P9/10; A61P11/06; A61P21/04; A61P25/00; A61P31/04; A61P31/18; A61P35/00; A61P37/00; C07K14/075; C12N7/00
Attorney, Agent or Firm:
Yoshio Kawaguchi (2 outside)