Title:
【発明の名称】血清アルブミンの除去/精製
Document Type and Number:
Japanese Patent JP2002536296
Kind Code:
A
Abstract:
A method for selectively enriching/removing a serum albumin from a mixture of other compounds by contacting said mixture with M-B-X where M is matrix, B the spacer and X the affinity ligand, with the provision that M may contain further groups X linked via a spacer. The characterizing feature is that the ligand X has been selected among serum albumin-binding structures complying with the formula (I) in which the free valence binds to the spacer B; R1-10 are selected from hydrogen, electron-withdrawing groups, such as halogens and lower alkyl groups (C1-10) that possibly are substituted with electron withdrawing groups, such as halogens; Z and Y are selected among oxygen, sulphur or nitrogen, with the provision that the nitrogen may carry a positive charge. A method for screening for ligands structures that, when attached to an affinity matrix, selectively bind serum albumin. The method has the characterizing feature that water-soluble compounds that exhibit a benzene ring fused to a 5-membered heterocycle containing two or three heteroatoms, preferably two, selected from nitrogen, oxygen and sulphur after having been attached to a matrix, preferably in the 2-position, are screened for selective binding to albumin.
Inventors:
Thor Legbury
Christel Elstrom
Christel Elstrom
Application Number:
JP2000589570A
Publication Date:
October 29, 2002
Filing Date:
December 20, 1999
Export Citation:
Assignee:
Amersham Biosciences Aktiebolag
International Classes:
A61K35/14; B01J20/281; C07K14/765; G01N30/88; G01N30/00; (IPC1-7): C07K14/765; A61K35/14; G01N30/00; G01N30/48
Foreign References:
| WO1998008603A1 | 1998-03-05 |
Attorney, Agent or Firm:
Aoyama Ryo (1 person outside)