Title:
【発明の名称】電気重合可能な薄膜とその製造方法とその使用法
Document Type and Number:
Japanese Patent JP2002539462
Kind Code:
A
Abstract:
An electrode and method of preparing an electrode by electropolymerizing a film on the conductive working surface of an electrode. The electrode is modified by reductive electropolymerization of a thin film of poly[Ru(vbpy)32+] or poly[Ru(vbpy)32+/vba] (vbpy=4-vinyl-4'methyl-2,2'-bipyridine and vba=p-vinylbenzoic acid) and the electrode is used for the electrochemical detection of aqueous GMP, poly[G], and surface immobilized single-stranded DNA probes. The film is formed from a co-polymer of a mediator such as Ru(vbpy)32+ and a functionalized moiety having a carboxylate group such as p-vinylbenzoic acid. A DNA probe is attached covalently to the carboxylate group via a carbodiimide reaction followed by amidation of an amino-linked single-stranded DNA. In the presence of these guanine containing moieties, a dramatic enhancement in the oxidative current for the Ru3+/2+ couple (present in the polymeric thin film) due to the catalytic oxidation of guanine is observed.
Inventors:
Thorpe, H Holden
Ontoco, Arin Sea
Ontoco, Arin Sea
Application Number:
JP2000605782A
Publication Date:
November 19, 2002
Filing Date:
February 04, 2000
Export Citation:
Assignee:
The University of North Carolina at Chapel Hill
International Classes:
C12M1/00; C12M1/40; C12N15/09; C12Q1/68; C12Q1/6816; C12Q1/6825; G01N27/30; G01N27/327; G01N33/53; G01N27/48; G01N33/566; G06F1/00; G06F21/00; C12Q; (IPC1-7): G01N27/30; C12M1/00; C12N15/09; C12Q1/68; G01N27/327; G01N33/53; G01N33/566
Attorney, Agent or Firm:
Okuyama Shoichi (2 outside)