Title:
【発明の名称】スクリーニング方法
Document Type and Number:
Japanese Patent JP2003504035
Kind Code:
A
Abstract:
The present invention provides a screening method for the identification of compounds which modulate an AMP-sensitive regulatory site on mitochondria comprising the steps of: a) contacting a test compound with mitochondria in the presence of a substrate for respiration in the presence of a buffer system; b) measuring an index of metabolic rate; and c) identifying compounds which modulate metabolic rate. Alternatively or additionally membrane potential can be measured. The invention also comprises a regulatory site for a mitochondrial proton leak which is related to the adenine nucleotide carrier, a binding assay and a functional assay.
Inventors:
Brand, Martin Dane
Cadeanas, Susana
Keith, Dateskinson
Robert Brian, The Johns
Cadeanas, Susana
Keith, Dateskinson
Robert Brian, The Johns
Application Number:
JP2001508369A
Publication Date:
February 04, 2003
Filing Date:
June 23, 2000
Export Citation:
Assignee:
Knorr GMBH
International Classes:
A61K45/00; A61P3/04; A61P43/00; C12N5/07; C12N5/071; C12Q1/02; G01N33/15; G01N33/50; (IPC1-7): C12Q1/02; A61K45/00; A61P3/04; A61P43/00; G01N33/15; G01N33/50
Attorney, Agent or Firm:
Yoshio Kawaguchi (5 outside)