Title:
【発明の名称】PNA-DNAキメラプローブを用いたテンプレート依存型ライゲーション
Document Type and Number:
Japanese Patent JP2003511059
Kind Code:
A
Abstract:
The invention provides methods, kits, and compositions for ligation of PNA-DNA chimeric probes and oligonucleotides when they are hybridized adjacently to template nucleic acids using ligases and ligation reagents. Structural requirements of the chimeras for ligation include 5 to 15 contiguous PNA monomer units, 2 or more contiguous nucleotides, and a 3' hydroxyl or 5' hydroxyl terminus. The chimera and/or oligonucleotide may be labelled with fluorescent dyes or other labels. The methods include, for example, oligonucleotide-ligation assays (OLA) and single nucleotide polymorphism detection.
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Inventors:
Egham, Michael
Chen, Kaif
Chen, Kaif
Application Number:
JP2001529456A
Publication Date:
March 25, 2003
Filing Date:
October 06, 2000
Export Citation:
Assignee:
Appraera Corporation
International Classes:
C12N15/09; C12Q1/68; G01N21/78; (IPC1-7): C12N15/09
Attorney, Agent or Firm:
Shusaku Yamamoto