Title:
【発明の名称】スルホヒドロラーゼ、相当するアミノ酸およびヌクレオチド配列、スルホヒドロラーゼ調製、方法およびその産物
Document Type and Number:
Japanese Patent JP2003517287
Kind Code:
A
Abstract:
A purified sulfohydrolase having a purity level based on total amount of protein of at least about 40 wt %. Isolated nucleic acid sequence and amino acid sequences. A process for purifying at least one sulfohydrolase, including subjecting an extract from seaweed to fractionation to obtain fractions; and subjecting at least one of the fractions to phenyl sepharose chromatography to obtain sepharose fractions containing at least one sulfohydrolase. An enzymatically modified compound which has been modified by an isolated sulfohydrolase having a purity level based on total amount of protein of at least about 40 wt %. A process of enzymatically modifying a sulfated compound, including combining at least one sulfohydrolase, having a purity level based on total amount of protein of at least about 40 wt %, with a sulfated compound form a reaction mixture; and incubating the reaction mixture to remove sulfate groups from the sulfated compound to form an enzymatically modified compound. A process of enzymatically modifying a sulfated compound, including incubating a first sulfohydrolase with a sulfated compound to remove sulfate groups from the sulfated compound to form an intermediate compound; and subsequently incubating the intermediate compound with a second sulfohydrolase to remove sulfate groups to form an enzymatically modified compound. A method for extracting one of nu- and mu-carrageenan from seaweed, including dispersing seaweed in a salt solution including K2CO3 to form a dispersion; filtering the dispersion to obtain a liquid; ultrafiltering the dispersion to remove salts; concentrating the liquid; adjusting the pH of the liquid to about 8 to 8.5; and precipitating one of nu- and mu-carrageenan from the liquid.
Inventors:
Jericho, Savan
De Reiter, Gerhard
Croare, bernard
Penninghoff bear
Philip, Philip
Richard, Odile
Brian Rudolph
De Reiter, Gerhard
Croare, bernard
Penninghoff bear
Philip, Philip
Richard, Odile
Brian Rudolph
Application Number:
JP2000616361A
Publication Date:
May 27, 2003
Filing Date:
May 09, 2000
Export Citation:
Assignee:
C/Kelco/APS
International Classes:
C08B37/00; C08B37/12; C12N9/14; C12N9/16; C12N15/55; C12P19/04; C12N15/09; (IPC1-7): C12N15/09; C08B37/00; C08B37/12; C12N9/16; C12P19/04
Attorney, Agent or Firm:
Kazuo Shamoto (5 outside)