Title:
【発明の名称】乳汁中にヒト顆粒球コロニー刺激因子を産生する形質転換ヤギ
Document Type and Number:
Japanese Patent JP2003520035
Kind Code:
A
Abstract:
A goat zygote (I) which comprises a nucleic acid construct containing a nucleotide sequence of a goat beta -casein promoter and a nucleic acid sequence encoding human granulocyte-colony stimulating factor (hG-CSF). Independent claims are also included for the following: (1) a method for preparing (I), comprising microinjecting a nucleic acid construct containing a nucleotide sequence of a goat beta-casein promoter and a nucleotide sequence encoding hG-CSF into an intact goat zygote; (2) a method (M1) for preparing an intact goat zygote, comprising synchronizing a female goat, superovulating the female goat, mating the superovulated female goat with a male goat and recovering a zygote from the mated female goat, characterized in that the synchronizing step is conducted by administering norgestomet and estradiol to the female goat, inserting an implant containing norgestomet to the female goat and removing the implant, and the superovulating step is conducted by administering to the female goat, sequentially at predetermined time intervals, a combined dose of pregnant mare serum gonadotropin (PMSG) and follicle stimulating hormone (FSH), divided doses of FSH, and a combined dose of FSH and human chorionic gonadotropin (hCG); (3) a transgenic goat producing milk containing hG-CSF, which is developed from (I); (4) a process for preparing the transgenic goat of (3) into a female goat and allowing the goat zygote to develop to term; (5) a method for producing hG-CSF, comprising producing milk from the transgenic goat of (3) and recovering the hG-CSF from the milk; and (6) a milk composition comprising hG-CSF, which is produced from the transgenic goat of (3); (7) hG-CSF produced by the method of (5). ACTIVITY : None given. No biological data given. MECHANISM OF ACTION : Human granulocyte-colony stimulating factor. No biological data given.
Inventors:
Jin Sung Won
Lee Doso
Song Tae Hoon
Choi In Young
Yo Ok-jeong
Ko Jun Ho
Ko Jashin
Shin Sante
Lee Chursan
Huang Nan Ju
Ko Dogong
Oh Combon
Park Jun Soon
Yong Ushik
Jen Guo Dong
Kim Seung Jun
Han Young Man
Lee Kyun Kwan
Lee Doso
Song Tae Hoon
Choi In Young
Yo Ok-jeong
Ko Jun Ho
Ko Jashin
Shin Sante
Lee Chursan
Huang Nan Ju
Ko Dogong
Oh Combon
Park Jun Soon
Yong Ushik
Jen Guo Dong
Kim Seung Jun
Han Young Man
Lee Kyun Kwan
Application Number:
JP2001552710A
Publication Date:
July 02, 2003
Filing Date:
January 26, 2001
Export Citation:
Assignee:
Hammi Farm. Seeo., LDT.
KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY
Korea Advanced Institute of Science and Technology
KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY
Korea Advanced Institute of Science and Technology
International Classes:
A01K67/027; C07K14/535; C07K14/55; C12N5/10; C12N15/09; C12N15/85; (IPC1-7): A01K67/027; C07K14/55; C12N5/10; C12N15/09
Attorney, Agent or Firm:
Kaoru Aoyama (3 people outside)