Title:
【発明の名称】細胞表面選択マーカー遺伝子
Document Type and Number:
Japanese Patent JP2003522525
Kind Code:
A
Abstract:
The present invention concerns a method of identifying genetically modified mammalian cells using a mutated protein-tyrosine kinase receptor (PTKR) as a selectable marker in mammalian cells. Particularly preferred mutated PTKR selective markers are mutated epidermal growth factor receptor (EGFR) family members, and muscle specific tyrosine kinase receptor (MuSK-R) family members. Further a method for the immunoselection of transduced mammalian cells is disclosed comprising retrovirally transducing mammalian cells with a nucleic acid sequence encoding a mutated EGFR, incubating the transduced cells with a marked antibody which recognizes and binds specifically to the mutated PTKR, and identifying the marked transduced cells.
Inventors:
Pippig Suzanne Degumer
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Beres gay bar
Application Number:
JP2001538537A
Publication Date:
July 29, 2003
Filing Date:
November 17, 2000
Export Citation:
Assignee:
Novartis Actien Gezel Shaft
International Classes:
C07K14/705; C12N9/12; C12N15/09; C12Q1/02; G01N33/15; G01N33/53; G01N33/50; G01N33/566; G01N33/569; (IPC1-7): C12N15/09; C12Q1/02; G01N33/15; G01N33/50; G01N33/53; G01N33/566
Domestic Patent References:
| JPH09501569A | 1997-02-18 |
Other References:
JPN6010033159, Ｇｅｎｅ Ｔｈｅｒａｐｙ，Ｖｏｌ．６，Ｎｏ．７（１９９９．Ｊｕｌ．）ｐ．１２２２−１２３０
JPN6010033160, Ｊ．Ｃｅｌｌ Ｂｉｏｌ．，Ｖｏｌ．１４６，Ｎｏ．５（１９９９．Ｓｅｐ．）ｐ．１１３３−１１４６
JPN6010033160, Ｊ．Ｃｅｌｌ Ｂｉｏｌ．，Ｖｏｌ．１４６，Ｎｏ．５（１９９９．Ｓｅｐ．）ｐ．１１３３−１１４６
Attorney, Agent or Firm:
Masahiro Abe