Title:
【発明の名称】高分子の三次元形状の決定方法
Document Type and Number:
Japanese Patent JP2003528288
Kind Code:
A
Abstract:
The present invention provides a fast and efficient method for determining the three-dimensional conformation of a protein. The steps of the method of the invention include: 1) formation of physical distance constraints, e.g., forming intramolecular chemical crosslinks of known size between residues of a protein; 2) enriching the number of the molecules that have intramolecular chemical crosslinks in the reaction pool, e.g., using size separation to remove proteins with intermolecular bonds; 3) exposing the enriched reaction pool to a protease that cuts the protein at specific sites to produce peptide fragments; 4) measuring the size of the peptide fragments to determine linkage sites with a certain spatial relationship in the protein; and 5) interpreting the data produced to determine spatial geometry and protein structure based on the deduced spatial relationship of the linkage sites. The information is preferably analyzed with aid from a computer system, which can be used to generate and/or analyze distance constraints between amino acids.
Inventors:
Gibson Bradford W.
Kunds Irwin Di.
Tan Nin
Dringer Gavin
Oscillo Connie M.
Hempel Judith See.
Taylor Eric
Kunds Irwin Di.
Tan Nin
Dringer Gavin
Oscillo Connie M.
Hempel Judith See.
Taylor Eric
Application Number:
JP2000620343A
Publication Date:
September 24, 2003
Filing Date:
May 26, 2000
Export Citation:
Assignee:
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA
International Classes:
G01N27/62; B01J20/283; C12N15/09; G01N30/06; G01N30/26; G01N30/72; G01N30/84; G01N30/88; G01N33/48; G01N33/68; G16B15/20; H01J49/04; (IPC1-7): G01N27/62; C12N15/09; G01N30/06; G01N30/48; G01N30/84; G01N30/88; G01N33/68
Attorney, Agent or Firm:
Takao Igarashi (3 outside)