Title:
バニロイド受容体についての改良リガンド結合アッセイ
Document Type and Number:
Japanese Patent JP2004511803
Kind Code:
A
Abstract:
The present invention describes an assay in which pH alters RTX affinity to VR1 receptors. The RTX affinity was increased with increasing pH from 4.1 to 8.6. Both the RTX affinity and apparent number of RTX binding sites were decreased with increasing pH from 8.6 to 10.6. The high pH may be causing the cell membranes to denature. These pH conditions (pH 8.0 to pH 9.0) provide a high signal to noise ratio, give a more robust assay and require use of less experimental materials.
Inventors:
Tuang, Sui Po
Application Number:
JP2002536547A
Publication Date:
April 15, 2004
Filing Date:
October 11, 2001
Export Citation:
Assignee:
Ortho-McNeill Pharmacy Incorporated
International Classes:
G01N33/53; G01N33/566; G01N33/543; (IPC1-7): G01N33/543; G01N33/53; G01N33/566
Attorney, Agent or Firm:
Heikichi Odashima