Title:
プリオン株を決定する方法
Document Type and Number:
Japanese Patent JP2004529314
Kind Code:
A
Abstract:
The present invention provides assays for identifying the levels of both protease sensitive and protease resistant conformers of PrPin a sample. In a preferred embodiment, the assay comprises determining levels of total PrPin a sample, subjecting the PrPfraction to treatment with a protease that selectively hydrolyzes the protease sensitive PrP(sPrP) conformers, and quantifying the levels of sPrPin the sample. The ability to detect sPrPallows early detection of prions, since the PrPin easily accessible biological samples such as blood is predominantly sPrP. The ratio of sPrPto rPrPalso allows the identification of a particular prion strain in an infected sample.
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Inventors:
Pulsinar Stanley Bee.
Sapphire
Sapphire
Application Number:
JP2002538170A
Publication Date:
September 24, 2004
Filing Date:
September 21, 2001
Export Citation:
Assignee:
The Regents of The University of California
International Classes:
A01N1/02; C12N11/00; G01N33/53; G01N33/531; G01N33/566; G01N33/68; (IPC1-7): G01N33/68; G01N33/53
Attorney, Agent or Firm:
Hatsushi Shimizu
Kazunori Hashimoto
Kazunori Hashimoto