Title:
内皮細胞発現パターンの評価法
Document Type and Number:
Japanese Patent JP2004533825
Kind Code:
A
Abstract:
To gain a better understanding of tumor angiogenesis, new techniques for isolating endothelial cells (ECs) and evaluating gene expression patterns were developed. When transcripts from ECs derived from normal and malignant colorectal tissues were compared with transcripts from non-endothelial cells, over 170 genes predominantly expressed in the endothelium were identified. Comparison between normal- and tumor-derived endothelium revealed 79 differentially expressed genes, including 46 that were specifically elevated in tumor-associated endothelium. Experiments with representative genes from this group demonstrated that most were similarly expressed in the endothelium of primary lung, breast, brain, and pancreatic cancers as well as in metastatic lesions of the liver. These results demonstrate that neoplastic and normal endothelium in humans are distinct at the molecular level, and have significant implications for the development of anti-angiogenic therapies in the future.
Inventors:
Carson-Walter Elinor
St. Croix Blood
Kin's Rare Kenneth W.
Voguelestein Bale
St. Croix Blood
Kin's Rare Kenneth W.
Voguelestein Bale
Application Number:
JP2002582213A
Publication Date:
November 11, 2004
Filing Date:
April 10, 2002
Export Citation:
Assignee:
THE JOHNS HOPKINS UNIVERSITY
International Classes:
A61K38/00; A61K39/395; A61K45/00; A61P9/10; A61P13/12; A61P17/06; A61P19/02; A61P29/00; A61P35/00; C07K14/47; C07K16/18; C07K16/30; C07K16/32; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N15/09; C12P21/08; G01N33/15; G01N33/53; G01N33/50; A61K39/00; (IPC1-7): C12N15/09; A61K38/00; A61K39/395; A61K45/00; A61P9/10; A61P13/12; A61P17/06; A61P19/02; A61P29/00; A61P35/00; C07K14/47; C07K16/18; C07K16/32; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12P21/08; G01N33/15; G01N33/50; G01N33/53
Attorney, Agent or Firm:
Hatsushi Shimizu
Kazunori Hashimoto
Koichi Niimi
Kazunori Hashimoto
Koichi Niimi