Title:
高スループット小分子薬物発見のための培養ヒト肥満細胞および好塩基球の産生
Document Type and Number:
Japanese Patent JP2005501552
Kind Code:
A
Abstract:
Provided are methods for producing and screening proliferated populations of CD34-negative progenitor cells, mucosal mast cells, connective tissue-type mast cells and basophil cells. The methods generate uniform proliferated populations of cells. The proliferated populations contain a uniform population of a size suitable for use in high throughput screening methods, for example, screening for agents that alter exocytosis. The invention includes screening the proliferated populations with at least one candidate bioactive agent, and evaluating the cells to detect a cell with an altered phenotype. The invention also includes isolating a candidate bioactive agent that causes the altered phenotype. Additionally, cells formed according to the described methods are also encompassed by the invention.
Inventors:
Rossi, Alexander Bee.
Application Number:
JP2003525600A
Publication Date:
January 20, 2005
Filing Date:
August 29, 2002
Export Citation:
Assignee:
Rigel Pharmaceuticals, Incorporated
International Classes:
C12N15/09; C12N5/02; C12N5/0787; C12Q1/02; (IPC1-7): C12N5/06; C12Q1/02
Attorney, Agent or Firm:
Atsushi Aoki
Takashi Ishida
Tetsuji Koga
Kazuhiro Nakamura
Masaya Nishiyama
Takashi Ishida
Tetsuji Koga
Kazuhiro Nakamura
Masaya Nishiyama