Title:
宿主細胞における生成物の生産方法
Document Type and Number:
Japanese Patent JP2005510999
Kind Code:
A
Abstract:
The invention provides methods and host cells for the production of ascorbic acid intermediates. The invention also provides host cells having a modification in a polynucleotide that uncouples the catabolic pathway from the oxidative pathway by deleting the encoding for an endogenous enzymatic activity that phosphorylates D-glucose at its 6 th carbon and/or a polynucleotide that has deleted the encoding for endogenous enzymatic activity that phosphorylates D-gluconate at its 6 th carbon. Such host cells are used for the production of products, such as, ascorbic acid intermediates. Nucleic acid and amino acid sequences with inactivated enzymatic activity which phosphorylates D-glucose at its 6 th carbon and inactivated enzymatic activity which phosphorylates D-gluconate at its 6 th carbon are provided.
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Inventors:
Dodge, Timothy Sea
Barre, Fernando
Barre, Fernando
Application Number:
JP2002579428A
Publication Date:
April 28, 2005
Filing Date:
April 04, 2002
Export Citation:
Assignee:
Genenco International Inc.
International Classes:
C12N15/09; C12N15/52; C12P7/40; C12P7/42; C12P7/46; C12P7/58; C12P7/60; C12P17/04; C12P19/02; (IPC1-7): C12N15/09; C12P7/40; C12P7/46
Attorney, Agent or Firm:
Yuzo Yamazaki
Toshio Iwahashi
Naoto Sugiyama
Shirogane Expo
Takako Hoshi
Toshiaki Akamatsu
Toshio Iwahashi
Naoto Sugiyama
Shirogane Expo
Takako Hoshi
Toshiaki Akamatsu