遺伝要素の組換え方法 - E.I.DU PONT DE NEMOURS AND COMPANY

Title:

遺伝要素の組換え方法

Document Type and Number:

Japanese Patent JP2005523690

Kind Code:

A

Abstract:

A method for the recombination of a gene is disclosed. The method involves the design of unpaired forward and reverse primers having homology to the 5′ end of one template and to the 3′ end of another template. Short primer extension periods results in a recombined template having paired 5′ and 3′ ends that can then be amplified. The amplified sample is devoid of any parental template.

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Inventors:

Milan, Gioseph
Tang, Xiao-Song

Application Number:

JP2003571431A

Publication Date:

August 11, 2005

Filing Date:

February 26, 2003

Export Citation:

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Assignee:

E.I.DU PONT DE NEMOURS AND COMPANY

International Classes:

C12N9/00; C12N9/88; C12N15/10; C12P21/02; C12N15/09; (IPC1-7): C12N15/09; C12N9/00; C12N9/88; C12P21/02

Foreign References:

US5965408A	1999-10-12
US6291242B1	2001-09-18
WO2001011071A2	2001-02-15

Attorney, Agent or Firm:

Heikichi Odashima

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