Title:
環境的条件または化学的条件への曝露による核酸2本鎖のコンフォメーション変化を酸化剤または反応剤の処理により検出する方法
Document Type and Number:
Japanese Patent JP2005526230
Kind Code:
A
Abstract:
A method of detecting a conformational change in a nucleic acid sample by treatment with oxidising agent for example KmnO4 to determine difference in formation or rate of formation of reaction product MnO2 or consumption or rate of consumption of KmnO4, which indicates conformational change in the test sample, in particular when the DNA is exposed to an environmental salt concentration and temperature or electrical current. The method investigates conformational change in the DNA, for example when DNA is exposed to an intercalating agent. The use of this method for investigating chemical compounds for conformational change in the DNA is also described. The conformational change is determined by colorimetric inorganic assay, by measuring absorbance by means of a spectrophotometer. The use of other oxidising agents besides permanganate comprising OsO4 or chromic acid or ozone gas, or peroxides or perbenzoic acids or electrical current, are described in the body of the specification.
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Inventors:
Cotton Richard G.
Bui Ting Thien
Beder Abdulkelim
Bui Ting Thien
Beder Abdulkelim
Application Number:
JP2003527419A
Publication Date:
September 02, 2005
Filing Date:
September 13, 2002
Export Citation:
Assignee:
Gene Batik Pty Limited
International Classes:
C07H21/00; C12Q1/68; G01N21/00; G01N33/52; G01N33/50; G01N33/53; G01N33/566; G01N33/84; (IPC1-7): G01N33/50; C12Q1/68; G01N33/53; G01N33/566
Attorney, Agent or Firm:
Hatsushi Shimizu
Kazunori Hashimoto
Koichi Niimi
Kazunori Hashimoto
Koichi Niimi