タンパク質ライブラリーのinsilico作成と選択

Japanese Patent JP2005526518

A

The present invention provides a methodology for efficiently generating and screening protein libraries for optimized proteins with desirable biological functions, such as improved binding affinity towards biologically and/or therapeutically important target molecules. The process is carried out computationally in a high throughput manner by mining the ever-expanding databases of protein sequences of all organisms, especially human. In one embodiment, a method for constructing a library of designed proteins, comprising the steps of: providing an amino acid sequence derived from a lead protein, the amino acid sequence being designated as a lead sequence; comparing the lead sequence with a plurality of tester protein sequences; and selecting from the plurality of tester protein sequences at least two peptide segments that have at least 15% sequence identity with the lead sequence, the selected peptide segments forming a hit library; and forming a library of designed proteins by substituting the lead sequence with the hit library. The library of designed proteins can be expressed in vitro or in vivo to produce a library of recombinant proteins that can be screened for novel or improved function(s) over the lead protein, such as an antibody against therapeutically important target.

Luo, Peach
Sea mark
Zhong, Pingu
One, Kairi
Khao, Ichen
Riu, Shengtian

JP2004508241A

September 08, 2005

May 20, 2003

Click for automatic bibliography generation  

Abu Maxis, Inc.

C12N15/09; G16B35/20; C07K16/00; C07K16/22; C12N1/15; C12N1/19; C12N1/21; C12N5/10; G16B20/30; G16B30/00; G16B30/10; G16B20/50; (IPC1-7): C12N15/09; C07K16/00

Atsushi Aoki
Takashi Ishida
Fukumoto product
Tetsuji Koga
Kazuhiro Nakamura
Masaya Nishiyama

Previous Patent: 分泌性ポリペプチドの検出

Next Patent: ヒダントインラセマーゼ