Title:
後方散乱を用いて血液細胞を分化するための方法及び装置
Document Type and Number:
Japanese Patent JP2005536742
Kind Code:
A
Abstract:
Blood cells of interest are readily distinguishable from other blood cells and look-a-like particles found in a blood sample by their back-scatter signature. A preferred method for differentiating platelets in a blood sample is to irradiate the cells and particles, one at a time, with a beam of radiation, and to detect back-scattered (reflected) radiation using a plurality of optical fibers to transmit the back-scattered radiation to a high-gain photodetector, e.g. a photomultiplier tube. Preferably, the back-scatter signal so obtained is combined with a second signal representing, for example, either the level of forward-scatter within a prescribed, relatively narrow angular range, or the level of side-scattered radiation, or the level of attenuation of the cell-irradiating beam caused by the presence of the irradiated cell or particle in the beam, or the electrical impedance of the irradiated cell or particle, to differentiate the cells of interest. The method and apparatus of the invention are particularly useful in differentiating platelets and basophils in a blood sample.
Inventors:
Kramer, Donald El.
Application Number:
JP2004530875A
Publication Date:
December 02, 2005
Filing Date:
August 06, 2003
Export Citation:
Assignee:
Coulter International Corporation
International Classes:
G01N33/49; G01N15/10; G01N15/14; G01N15/00; (IPC1-7): G01N15/14; G01N33/49
Attorney, Agent or Firm:
Atsushi Aoki
Jun Tsuruta
Tetsuro Shimada
Kurachi Yasuyuki
Shimichi Akihisa
Masaya Nishiyama
Jun Tsuruta
Tetsuro Shimada
Kurachi Yasuyuki
Shimichi Akihisa
Masaya Nishiyama
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