Title:
親水性化学発光アクリジニウムラベル化剤
Document Type and Number:
Japanese Patent JP2005536748
Kind Code:
A
Abstract:
In accordance with the present invention, it has been discovered that introduction of hydrophilic sulfoalkyl substituents and/or hydrophilic linkers derived from homocysteic acid, cysteic acid, glycine peptides, tetraethylene oxide, and the like, offset the hydrophobicity of the acridinium ring system to produce a more soluble label which can be attached to an antibody at higher loading before precipitation and aggregation problems are encountered. Additional compounds described herein contain linkers derived from short peptides and tetraethylene oxide which increase aqueous solubility due to hydrogen bonding with water molecules. The present invention also embraces reagents for multiple acridinium labeling for signal amplification composed of a peptide bearing several acridinium esters with sulfonate groups at regularly spaced intervals for increased solubility. The invention also embraces assays employing the above-described compounds.
Inventors:
Evangelista, Ramon, A
Martyr, Martha
Martyr, Martha
Application Number:
JP2004531077A
Publication Date:
December 02, 2005
Filing Date:
August 18, 2003
Export Citation:
Assignee:
Quest Diagnostics Investments Inc.
International Classes:
G01N33/532; C07D219/04; C07D401/12; C09B15/00; C09K11/07; G01N21/78; G01N33/533; G01N33/58; (IPC1-7): G01N21/78; C07D219/04; C07D401/12; C09K11/07; G01N33/532
Domestic Patent References:
| JP2003050204A | 2003-02-21 | |||
| JPH03172522A | 1991-07-25 | |||
| JPH02133469A | 1990-05-22 | |||
| JPH10503169A | 1998-03-24 | |||
| JPH08504751A | 1996-05-21 |
Foreign References:
| WO2000031543A1 | 2000-06-02 | |||
| WO2000053809A2 | 2000-09-14 | |||
| EP0442372A1 | 1991-08-21 |
Attorney, Agent or Firm:
Seiji Ohno
Koji Morita
Reiko Tanaka
Ken Kitano
Koji Morita
Reiko Tanaka
Ken Kitano