Title:
ランピルナーゼ変異体をコードする核酸、および、それらの製造方法
Document Type and Number:
Japanese Patent JP2006512082
Kind Code:
A
Abstract:
Ribonuclease DNA coding for an amino acid sequence beginning with a residue of glutamine is introduced into a vector of pET22b(+) plasmid to form recombinant plasmid DNA that begins with a Pel B leader sequence. The recombinant plasmid DNA is used to transform an E.coli BL21(DE3) host. Signal peptidase enzyme present in the host cell cleaves the pelB leader sequence during signal processing and thereby allows the glutamine residue to autocyclize to pyroglutamic acid. In this way, the Ribonuclease can be produced directly, i.e. without a separate step of cleaving the initial N-terminal methionine residue.
Inventors:
Shirendra Kay Saxena
Application Number:
JP2004565610A
Publication Date:
April 13, 2006
Filing Date:
December 20, 2003
Export Citation:
Assignee:
Al Huasel Corporation
International Classes:
C12N15/09; C12N1/21; C12N9/22
Attorney, Agent or Firm:
Chika Takagi
Junji Yuda
Shoji Miwa
Junji Yuda
Shoji Miwa