Title:
メソ置換シアニン色素標識化試薬
Document Type and Number:
Japanese Patent JP2006523257
Kind Code:
A
Abstract:
Disclosed are cyanine dyes having meso-substituents in the polymethine chain linking the heterocyclic ring systems. The dyes are of formula (1): in which groups R3 and R4 are attached to the Z1 ring structure and groups R5 and R6 are attached to the Z2 ring structure, and n=1, 2 or 3; Z1 and Z2 independently represent the atoms necessary to complete one ring, or two fused ring aromatic or heteroaromatic systems, each ring having five or six atoms selected from carbon atoms and optionally no more than two atoms selected from oxygen, nitrogen and sulphur; X and Y are the same or different and are selected from oxygen, sulphur, -CH-CH- and the group: at least one of groups R1, R2, R3, R4, R5, R6 (and R8 and R9 if present) is the group -E-F where E is a spacer group and F is a target bonding group; one of groups R7 is selected from -CN, -Cl, -F, -CF3 and -C(O)R10 wherein R10 is selected from H, C1-C6 alkyl and aryl. Preferred group R7 is meso-substituted -CN, which confers an unexpected hypsochromic shift of approximately 40 nm in the emission spectrum, when compared with the corresponding unsubstituted analogue. The dyes are useful for labelling and detecting a variety of target molecules.
Inventors:
Cheng, Chun-Yang
Bear,sieve
Bear,sieve
Application Number:
JP2006507193A
Publication Date:
October 12, 2006
Filing Date:
March 12, 2004
Export Citation:
Assignee:
GE Healthcare Bioscience Corporation
International Classes:
C09B23/00; C07D209/18; C07D209/24; C09B23/01; C09B23/02; G01N21/78; G01N33/58
Domestic Patent References:
| JPS6289769A | 1987-04-24 | |||
| JPH09230539A | 1997-09-05 | |||
| JP2002264504A | 2002-09-18 | |||
| JP2002128753A | 2002-05-09 |
Foreign References:
| DE19957007A1 | 2001-05-31 | |||
| CA2425701A1 | 2002-04-25 | |||
| US6004536A | 1999-12-21 | |||
| US20020064794A1 | 2002-05-30 | |||
| US6048982A | 2000-04-11 | |||
| US20030135058A1 | 2003-07-17 | |||
| US20020156288A1 | 2002-10-24 |
Attorney, Agent or Firm:
Kenichi Matsumoto
Hirokazu Ogura
Nobukazu Ito
Toshihisa Kurokawa
Hirokazu Ogura
Nobukazu Ito
Toshihisa Kurokawa