Title:
ヒト角膜内皮細胞および角膜細胞移植術のための細胞の取得および培養方法
Document Type and Number:
Japanese Patent JP2007508015
Kind Code:
A
Abstract:
A method for isolation of human corneal endothelial cells by non-enzymatic harvesting and culturing of endothelial cells in vitro from critical density in a system consisting of extracellular matrix protein coated plastic dishes and growth factor enriched culture medium are used to generate expanded sub-populations of endothelial cells for cell replacement procedures or transplantation in denuded donor corneas. Special procedures are included in the process for the removal of the native corneal endothelium from the donor corneas and the seeding of the culture corneal endothelial cells onto the denuded corneas for transplantation purposes.
Inventors:
Ge Ming LUI
Application Number:
JP2006534292A
Publication Date:
April 05, 2007
Filing Date:
October 07, 2004
Export Citation:
Assignee:
Ge Ming LUI
CELLULAR BIOENGINEERING, INC.
CELLULAR BIOENGINEERING, INC.
International Classes:
A61L27/00; A61L27/38; C12M1/00; C12M3/00; C12N5/071
Domestic Patent References:
| JP2002078723A | 2002-03-19 |
Foreign References:
| WO2003044045A2 | 2003-05-30 |
Attorney, Agent or Firm:
Mitsuo Tanaka
Michiko Matsutani
Mina Shiga
Sakurai Yoko
Michiko Matsutani
Mina Shiga
Sakurai Yoko