Title:
マイクロアレイハイブリダイゼーション装置
Document Type and Number:
Japanese Patent JP2007515627
Kind Code:
A
Abstract:
A novel hybridization device that improves the efficiency and consistency of microarray hybridization reactions by achieving a greater degree of internal mixing of target solution. The device provides a gasket-and-cover-type chamber wherein solution mixing is achieved by the creation of a multitude of microbubbles. One or more of the inner walls that define the chamber contain bubble-rupturing elements that extend into the chamber and terminate in sharp edges. They are typically located on opposite sides of a rectangular chamber and are pointed in a direction opposing bubble movement. Their interference with larger bubbles causes their breakup into microbubbles which travel separate and distinct paths as a result of external agitation and thereby provide improved solution mixing that results in a uniform distribution of target molecules to the probe molecules bound to the substrate. The sensitivity and consistency of the hybridization reaction is significantly increased.
Inventors:
Soonkap Hahn
Jobe steadman
Pavel Zinberg
Tim Watanas Kuru
Yevdit Gerassy
Jobe steadman
Pavel Zinberg
Tim Watanas Kuru
Yevdit Gerassy
Application Number:
JP2006541216A
Publication Date:
June 14, 2007
Filing Date:
November 03, 2004
Export Citation:
Assignee:
Biocept Inc.
International Classes:
B01F33/40; G01N35/02; B01J19/00; B01L3/00; C12Q1/68; G01N35/00; G01N37/00; C40B60/14; G01N33/53
Attorney, Agent or Firm:
Yoshikazu Tani
Kazuo Abe
Kazuo Abe