ナノ-PCR:核酸増幅及び検出のための方法及び装置

Japanese Patent JP2008500029

A

Methods, devices, and compositions are described that provide for amplification of nucleic acid sequences without reliance upon temperature cycling, thus freeing the methods from conventional benchtop thermal cycling devices. Denaturation of double stranded nucleic acids, primer annealing, and precision control over primer extension by polymerase can be accomplished by applying stress to a nucleic acid. These methods can provide one ore more benefits over conventional PCR methods including: precision control over the PCR process; generally improved fidelity; improved accuracy over problematic sequences such as GC-rich or tandem repeat regions; greater sequence length; increased reaction yield; reduced experimental time; greater efficiency; lower cost; greater portability; and, robustness to various environmental parameters, such as temperature, pH, and ionic strengths.

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JP3192419	INDUSTRIAL APPLICABILITY The serine protease mutant strain of the chymotrypsin superfamily resistant to inhibition by an inhibitor of the same origin and a gene encoding the same, and a serine protease inhibitor mutant strain and a gene encoding the same.
WO/1995/007995	ADENO-ASSOCIATED VIRAL (AAV) LIPOSOMES AND METHODS RELATED THERETO

Goel, Anita

JP2007513358A

January 10, 2008

May 13, 2005

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GOEL, Anita

C12N15/09; C12M1/00; C12Q1/68; G01N33/53; G01N33/569; G01N37/00

JP2003507026A

2003-02-25

US4965188A	1990-10-23
US5753439A	1998-05-19

Yasunori Otsuka
Shiro Takayanagi
Yasuhiro Otsuka
Shuji Kimura
Osamu Shimoyama

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