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Title:
ナノ-PCR:核酸増幅及び検出のための方法及び装置
Document Type and Number:
Japanese Patent JP2008500029
Kind Code:
A
Abstract:
Methods, devices, and compositions are described that provide for amplification of nucleic acid sequences without reliance upon temperature cycling, thus freeing the methods from conventional benchtop thermal cycling devices. Denaturation of double stranded nucleic acids, primer annealing, and precision control over primer extension by polymerase can be accomplished by applying stress to a nucleic acid. These methods can provide one ore more benefits over conventional PCR methods including: precision control over the PCR process; generally improved fidelity; improved accuracy over problematic sequences such as GC-rich or tandem repeat regions; greater sequence length; increased reaction yield; reduced experimental time; greater efficiency; lower cost; greater portability; and, robustness to various environmental parameters, such as temperature, pH, and ionic strengths.

Inventors:
Goel, Anita
Application Number:
JP2007513358A
Publication Date:
January 10, 2008
Filing Date:
May 13, 2005
Export Citation:
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Assignee:
GOEL, Anita
International Classes:
C12N15/09; C12M1/00; C12Q1/68; G01N33/53; G01N33/569; G01N37/00
Domestic Patent References:
JP2003507026A2003-02-25
Foreign References:
US4965188A1990-10-23
US5753439A1998-05-19
Attorney, Agent or Firm:
Yasunori Otsuka
Shiro Takayanagi
Yasuhiro Otsuka
Shuji Kimura
Osamu Shimoyama