Title:
インシリコ分析に基づく菌株の改良方法
Document Type and Number:
Japanese Patent JP2008527991
Kind Code:
A
Abstract:
The present invention is related to a method for improving a strain on the basis of in silico analysis, in which it compares the genomic information of a target strain for producing a useful substance to the genomic information of a strain overproducing the useful substance so as to primarily screen genes unnecessary for the overproduction of the useful substance, and then to secondarily screen genes to be deleted through performing simulation with metabolic flux analysis. According to the present invention, an improved strain can be effectively constructed by the metabolic and genetic engineering approach comprising comparatively analyzing the genomic information of a target strain for producing a useful substance and the genomic information of a strain producing a large amount of the useful substance to screen candidate genes and performing in silico simulation on the screened candidate genes to select a combination of genes to be deleted, which shows an improvement in the production of the useful substance. Accordingly, the time, effort and cost required for an actual wet test can be significantly reduced.
Inventors:
Issanyup
Kim Taeyeon
Itonyu
Issanjun
Kim Taeyeon
Itonyu
Issanjun
Application Number:
JP2007552047A
Publication Date:
July 31, 2008
Filing Date:
May 23, 2005
Export Citation:
Assignee:
ELECTRONICS AND TELECOMMUNICATIONS RESEARCH INSTITUTE
International Classes:
C12N15/09; C12N1/21; C12P7/46
Other References:
JPN6010028772, Ｂｉｏｔｅｃｈｎｏｌ． Ｂｉｏｅｎｇ．， ８４［６］ （２００３） ｐ．６４７−６５７
Attorney, Agent or Firm:
Kenichi Morita
Kenjiro Yamaguchi
Kenjiro Yamaguchi