Title:
最適化されたTRPM8核酸配列、ならびにTRPM8モジュレーターを同定するための、細胞に基づいたアッセイおよび検査キットにおけるそれらの使用
Document Type and Number:
Japanese Patent JP2009511049
Kind Code:
A
Abstract:
Modified human TRPM8 nucleic acid sequences which are efficiently expressed in human cells and cell-based assays and test kits containing same are provided. These assays identify TRPM8 modulators using cells that express a modified human TRPM8 nucleic acid sequence according to the invention, wherein said sequence has been modified relative to a wild-type human TRPM8 nucleic acid sequence in order to optimize ion channel expression in desired cells. Assays using these modified TRPM8 sequences have been shown to identify compounds that modulate the human TRPM8 ion channel better or comparably to known coolants such as menthol and icilin.
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Inventors:
Servant guy
Blast, pole
Brian Moyer
Lou, Min
Fernando Ecevalli
Dahan, david
Zoller, Mark
Mark williams
Rachel, Kimichi
Camder, Pounit
Ditchoun, Tanya
Andrew Patron
Blast, pole
Brian Moyer
Lou, Min
Fernando Ecevalli
Dahan, david
Zoller, Mark
Mark williams
Rachel, Kimichi
Camder, Pounit
Ditchoun, Tanya
Andrew Patron
Application Number:
JP2008535572A
Publication Date:
March 19, 2009
Filing Date:
October 04, 2006
Export Citation:
Assignee:
Cenomics Incorporated
International Classes:
C12N15/09; C12N5/10; C12Q1/02; G01N21/78; G01N33/15; G01N33/50
Attorney, Agent or Firm:
Kazuo Shamoto
Shinjiro Ono
Yasushi Kobayashi
Akio Chiba
Hiroyuki Tomita
Shinjiro Ono
Yasushi Kobayashi
Akio Chiba
Hiroyuki Tomita