Title:
低分子量ヒアルロン酸の生成
Document Type and Number:
Japanese Patent JP2009526529
Kind Code:
A
Abstract:
The present invention relates to methods for producing a hyaluronic acid with a desired average molecular weight in the range of 20,000-800,000 Dalton, the methods comprising the steps of: (a) cultivating a recombinant Bacillus host cell at a first temperature conducive to its growth, wherein the Bacillus host cell comprises a nucleic acid construct comprising a hyaluronan synthase encoding sequence operably linked to a promoter sequence foreign to the hyaluronan synthase encoding sequence; (b) then cultivating the recombinant Bacillus host cell of step (a) at a second temperature higher than the first temperature of step (a) under conditions suitable for production of the hyaluronic acid, whereby the Bacillus host cell produces hyaluronic acid with a desired average molecular weight in the range of 20,000-800,000 Dalton; and (c) recovering the hyaluronic acid.
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Inventors:
Stocks, Stuart M.
Brown, steven
Brown, steven
Application Number:
JP2008554595A
Publication Date:
July 23, 2009
Filing Date:
February 15, 2007
Export Citation:
Assignee:
Novozymes Biopolymer Actiesel Scab
International Classes:
C12P19/26; C12N15/09
Domestic Patent References:
| JP2005525091A | 2005-08-25 | |||
| JP2005514059A | 2005-05-19 | |||
| JP2005269935A | 2005-10-06 |
Foreign References:
| WO2003095658A1 | 2003-11-20 |
Other References:
JPN6012024645; ＡＰＰＬＩＥＤ ＡＮＤ ＥＮＶＩＲＯＮＭＥＮＴＡＬ ＭＩＣＲＯＢＩＯＬＯＧＹ ｖｏｌ．７１ ｎｏ．７, 2005, ｐｐ．３７４７-３７５２
JPN6012024646; ＡＰＰＬＩＥＤ ＡＮＤ ＥＮＶＩＲＯＮＭＥＮＴＡＬ ＭＩＣＲＯＢＩＯＬＯＧＹ ｖｏｌ．６３ ｎｏ．７, 1997, ｐｐ．２７５９-２７６４
JPN6012024647; 化学工学論文集 ｖｏｌ．１８ ｎｏ．５, 1992, ｐｐ．６８４-６９２
JPN6012024646; ＡＰＰＬＩＥＤ ＡＮＤ ＥＮＶＩＲＯＮＭＥＮＴＡＬ ＭＩＣＲＯＢＩＯＬＯＧＹ ｖｏｌ．６３ ｎｏ．７, 1997, ｐｐ．２７５９-２７６４
JPN6012024647; 化学工学論文集 ｖｏｌ．１８ ｎｏ．５, 1992, ｐｐ．６８４-６９２
Attorney, Agent or Firm:
Atsushi Aoki
Takashi Ishida
Fukumoto product
Tetsuji Koga
Kazuhiro Nakamura
Yoichi Watanabe
Masaru Nakajima
Takashi Ishida
Fukumoto product
Tetsuji Koga
Kazuhiro Nakamura
Yoichi Watanabe
Masaru Nakajima