前庭水管拡大症候群性難聴に関連するPDS遺伝子の突然変異を体外で特定するためのプライマーの製造方法及びその使用

Japanese Patent JP2009528036

A

The present invention relates to a method of preparing a primer used for determining the IVS7-2A†’G site mutation involved in the large vestibular aqueduct syndrome. The method comprises the steps of : designing a primer pair which may introduce any base substitutive mutation in the region of 1-13 bases between upstream and downstream including the IVS7-2 mutation site (A†’G) based upon the IVS7-2 A†’ G mutation site in the PDS gene, so as to obtain a new restriction site which may be used for distinguishing IVS7-2 wild type A site and mutant G site in the amplification products. The present invention further relates to the primers prepared according to this method, the kit or reagent or identical product comprising the primers, and a method for determining the IVS7-2A†’G site mutation of large vestibular aqueduct syndrome with said primers in vitro.

JIN ZHENGCE
Korea East
Park

JP2008556634A

August 06, 2009

January 24, 2007

Click for automatic bibliography generation  

JIN ZHENGCE

C12N15/09; C12Q1/68

JPN6012023357; Ｊ．　Ｍｅｄ．　Ｇｅｎｅｔ． Ｖｏｌ．４０, 2003, ｐｐ．２４２-２４８
JPN6012023359; Ｊ．　Ｃｌｉｎ．　Ｅｎｄｏｃ． Ｖｏｌ．８６，　Ｎｏ．８, 2001, ｐｐ．３９０７-３９１１
JPN6012023360; Ｈｕｍａｎ　Ｍｕｔａｔｉｏｎ Ｖｏｌ．１７, 2001, ｐｐ．４０３-４１１
JPN6012023361; Ｎａｔｕｒｅ　Ｇｅｎｅｔｉｃｓ Ｖｏｌ．１７，　Ｎｏ．４, 1997, ｐｐ．４１１-４２２
JPN6012023349; Ｈｅａｒｉｎｇ　Ｒｅｓｅａｒｃｈ Ｖｏｌ．１９９, 2005, ｐｐ．２２-３０
JPN6012023352; 古庄敏行ほか編: 臨床ＤＮＡ診断法 , 1995, ｐｐ．１７０-１７７, 金原出版株式会社
JPN6012023354; Ｔｈｅ　ＬＡＮＣＥＴ Ｖｏｌ．３３７, 1991, ｐｐ．１１１５-１１１８
JPN6012023355; Ｎｕｃｌｅｉｃ　Ａｃｉｄｓ　Ｒｅｓｅａｒｃｈ Ｖｏｌ．１７，　Ｎｏ．９, 1989, ｐ．３６０６

Koichi Washida

Previous Patent: 新規なバイオマスによる液体燃料の製造方法

Next Patent: 核酸増幅用基材