Title:
混合試料の特性決定法
Document Type and Number:
Japanese Patent JP2009531037
Kind Code:
A
Abstract:
A method is provided for characterizing a mixed sample having at least two particles with nucleic acids from different individuals, where each particle has nucleic acid from one or more individuals, in particular for the quantitative determination of the absolute and/or relative copy number of a predetermined sequence of an individual, of which nucleic acid is present in the mixed sample, having the steps: a) isolating the particles and applying at least two individual particles to a substrate, where each of the at least two particles is deposited in each case individually to a hydrophilic reaction site, surrounded by a hydrophobic zone, of the substrate in a volume of less than 10 μl so that precisely one particle is present per reaction site, b) analysis of at least two of the particles deposited to the substrate at the reaction site of the substrate to assign each of the particles to individuals from the mixed sample by genotyping, where at least 80% of the particles analysed are to be assigned to an individual, and c) further characterization of the analysed particles. Moreover, a kit which is suitable in particular for carrying out this method, is also provided.
Inventors:
Christoph Gauer
Man Wolfgang
Man Wolfgang
Application Number:
JP2009501879A
Publication Date:
September 03, 2009
Filing Date:
February 15, 2007
Export Citation:
Assignee:
ADVARITICS AG
International Classes:
C12Q1/68; G01N37/00; G01N33/543
Attorney, Agent or Firm:
Hiroshi Maeda
Hiroshi Takeuchi
Takahisa Shimada
Yuji Takeuchi
Katsumi Imae
Atsushi Fujita
Kazunari Ninomiya
Tomoo Harada
Iseki Katsumori
Seki Kei
Yasuya Sugiura
Hiroshi Takeuchi
Takahisa Shimada
Yuji Takeuchi
Katsumi Imae
Atsushi Fujita
Kazunari Ninomiya
Tomoo Harada
Iseki Katsumori
Seki Kei
Yasuya Sugiura