Title:
断片ライゲーションによる再構成
Document Type and Number:
Japanese Patent JP2010500040
Kind Code:
A
Abstract:
The present invention provides methods for preparing polynucleotide variants. In one aspect of the invention, the method comprises: a) exposing a pool of two or more related polynucleotides to at least one nicking enzyme, where at least some of the polynucleotides are partially and/or fully double stranded, b) removing, and/or inhibiting the activity of, the at least one nicking enzyme, c) denaturing the polynucleotides, d) allowing the denatured polynucleotides to form at least partially double stranded polynucleotides, e) exposing the double stranded polynucleotides formed in step d) to a ligase. Also provided are methods of making a polynucleotide and/or polypeptide having a desired property.
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Inventors:
Christopher Wayne Copan
Robin Jay Russell
John G. Oak Shot
Colin Scott
Robin Jay Russell
John G. Oak Shot
Colin Scott
Application Number:
JP2009524047A
Publication Date:
January 07, 2010
Filing Date:
August 15, 2007
Export Citation:
Assignee:
COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANISATION
International Classes:
C12N15/09; C07K2/00; C12P19/34; C40B40/08
Attorney, Agent or Firm:
Masatake Shiga
Takashi Watanabe
Yasuhiko Murayama
Shinya Mitsuhiro
Takashi Watanabe
Yasuhiko Murayama
Shinya Mitsuhiro