Title:
結合分子
Document Type and Number:
Japanese Patent JP2010503659
Kind Code:
A
Abstract:
The present invention relates to methods for engineering VH domains to improve their solubility and stability. The invention provides for the incorporation of defined amino acid substitutions based on 3-D structural information into the V segments of a heavy chain locus, expressing the locus in a non-human mammal and selecting soluble VH domains. Further stabilising or solubilising mutations maybe introduced as a result affinity maturation during B-cell maturation in vivo.
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Inventors:
Floss felt, franklin heraldus
Janssens, Rehart Wilhelm
Dravek, Duvlavka
Janssens, Rehart Wilhelm
Dravek, Duvlavka
Application Number:
JP2009527923A
Publication Date:
February 04, 2010
Filing Date:
September 18, 2007
Export Citation:
Assignee:
Erasmus University Medical Center Rotterdam
International Classes:
C07K16/00; C12N15/09; C12P21/08
Attorney, Agent or Firm:
Shoichi Okuyama
Arihara Koichi
Matsushima Tetsuo
Hidefumi Kawamura
Naomi Yoshida
Ayako Nakamura
Masayuki Okamoto
Fukagawa Eri
Satoshi Morimoto
Arihara Koichi
Matsushima Tetsuo
Hidefumi Kawamura
Naomi Yoshida
Ayako Nakamura
Masayuki Okamoto
Fukagawa Eri
Satoshi Morimoto