Title:
肺炎連鎖球菌3型多糖体の精装
Document Type and Number:
Japanese Patent JP2010505963
Kind Code:
A
Abstract:
The present invention provides improved methods for the reduction or removal of protein impurities from a complex cellular Streptococcus pneumoniae lysate or centrate comprising serotype 3 polysaccharides involving steps relating to post-lysis heating or pH adjustment. In certain methods, the lysate is heated for a time and at a temperature sufficient to denature proteins present in the lysate and cause their aggregation and precipitation. In one embodiment, the lysate is heated to at least 60° C. for at least 30 minutes to cause protein aggregation and precipitation, more particularly about 60° C. to about 70° C. for about 30 to about 50 minutes, and even more particularly about 65° C. for about 40 minutes. In other methods, the pH of the lysate or centrate is increased to at least 8.0 to improve filterability, more particularly about 8.0 to 8.4, and even more particularly about 8.2. In further methods, heating and pH adjustment steps are combined to cause the aggregation and precipitation of proteins as well as to improve filterability of the lysates or centrates. In other methods, the pH of the lysate or centrate is lowered to about 3.0 to about 5.0 to cause protein aggregation and precipitation. Such methods allow for the production of substantially purified serotype 3 polysaccharide-containing lysates or centrates.
Inventors:
Brian Douglas Barrer
Eric Heller Hughes
Tsu-Shun Lee
Eric Heller Hughes
Tsu-Shun Lee
Application Number:
JP2009532526A
Publication Date:
February 25, 2010
Filing Date:
October 09, 2007
Export Citation:
Assignee:
Weiss LRC
International Classes:
A61K39/09; A61K35/74; A61K39/00; B01D61/14; B01D61/16
Domestic Patent References:
| JPH0565300A | 1993-03-19 | |||
| JP2002539273A | 2002-11-19 |
Foreign References:
| WO2007084856A2 | 2007-07-26 |
Attorney, Agent or Firm:
Mitsuo Tanaka
Hiroshi Yamazaki
Tadayuki Motoyama
Kenji Tomita
Hiroshi Yamazaki
Tadayuki Motoyama
Kenji Tomita