Title:
遺伝子操作されたウサギ抗体可変ドメイン及びその使用
Document Type and Number:
Japanese Patent JP2010525808
Kind Code:
A
Abstract:
The present invention relates to methods for screening and producing polypeptides that immunospecifically bind to an antigen, which polypeptides comprise binding domains that are derived from rabbit immunoglobulin. Using rabbit antibody heavy-chain or light-chain scaffolds, the methods of the invention allow identification of novel CDR loops and framework regions that confer enhanced stability and/or affinity to isolated immunoglobulin variable domains, in particular, relative to those derived from rodent antibodies. The enhanced stability and/or affinity of the variable domains of the invention permit their use in the production research tools or therapeutic immunospecific polypeptides, including single domain immunospecific polypeptides, i.e., comprising one of a VH or VL domain.
Inventors:
Brass Goncalves Joanne Manuel
Castagneira Aires da Silva Frederico Nuno
Castagneira Aires da Silva Frederico Nuno
Application Number:
JP2010506111A
Publication Date:
July 29, 2010
Filing Date:
April 30, 2008
Export Citation:
Assignee:
Technophage, Investigasan Edecenvolviment Mbiotechnologya, SA
International Classes:
C12P21/08; C07K16/00; C12N15/09
Domestic Patent References:
| JP2005535341A | 2005-11-24 |
Other References:
JPN6013024247; Ｊ． Ｂｉｏｌ． Ｃｈｅｍ．， （２０００）， ２７５， ［１８］， ｐ．１３６６８-１３６７６
JPN6013024248; Ｇｅｎｅ， （１９９３）， １２６， ［１］， ｐ．１０９-１１３
JPN6013024249; Ｍｉｃｒｏｂ． Ｐａｔｈｏｇ．， （２００４）， ３７， ［２］， ｐ．９５-１０５
JPN6013024248; Ｇｅｎｅ， （１９９３）， １２６， ［１］， ｐ．１０９-１１３
JPN6013024249; Ｍｉｃｒｏｂ． Ｐａｔｈｏｇ．， （２００４）， ３７， ［２］， ｐ．９５-１０５
Attorney, Agent or Firm:
Masaki Hirota