Title:
PURO-DHFR四機能性マーカー、及びタンパク質の生産におけるその使用
Document Type and Number:
Japanese Patent JP2010528641
Kind Code:
A
Abstract:
This invention relates to industrial production of proteins. More specifically, the invention relates to the res-DHFR surrogate marker, which corresponds to a fusion between DHFR and a protein conferring resistance to a toxic compound or conferring a metabolic advantage. The invention further relates to the use of res-DHFR for screening cells for high expression of a protein of interest. The invention is illustrated by the Puro-DHFR surrogate marker, which corresponds to a fusion between the puromycin N-acetyltransferase and dihydrofolate reductase (DHFR).
Inventors:
Cobb, Michelle
Dupra, Philip
Dupra, Philip
Application Number:
JP2010510821A
Publication Date:
August 26, 2010
Filing Date:
June 06, 2008
Export Citation:
Assignee:
Merck Serono Society Anonym
International Classes:
C12N15/09; C12N5/10; C12N9/02; C12N9/10; C12P21/02; C12Q1/06; C12Q1/68; C07K19/00
Domestic Patent References:
| JP2003504059A | 2003-02-04 | |||
| JP2006512061A | 2006-04-13 | |||
| JP2006512061A | 2006-04-13 | |||
| JP2003504059A | 2003-02-04 |
Foreign References:
| WO2007023184A1 | 2007-03-01 | |||
| WO2007023184A1 | 2007-03-01 |
Other References:
JPN5010007280; ＭＩＬＬＥＲ ＬＡＷＲＥＮＣＥ Ｗ: ＮＡＴＵＲＥ ＭＥＴＨＯＤＳ Ｖ２ Ｎ４, 200504, Ｐ２５５-２５７
JPN5010007280; ＭＩＬＬＥＲ ＬＡＷＲＥＮＣＥ Ｗ: ＮＡＴＵＲＥ ＭＥＴＨＯＤＳ Ｖ２ Ｎ４, 200504, Ｐ２５５-２５７
JPN5010007280; ＭＩＬＬＥＲ ＬＡＷＲＥＮＣＥ Ｗ: ＮＡＴＵＲＥ ＭＥＴＨＯＤＳ Ｖ２ Ｎ４, 200504, Ｐ２５５-２５７
Attorney, Agent or Firm:
Atsushi Aoki
Takashi Ishida
Fukumoto product
Tetsuji Koga
Yoichi Watanabe
Kazuhiro Nakamura
Masaru Nakajima
Takashi Ishida
Fukumoto product
Tetsuji Koga
Yoichi Watanabe
Kazuhiro Nakamura
Masaru Nakajima