Title:
所望のタンパク質を高レベルで発現する安定細胞株を生産する方法
Document Type and Number:
Japanese Patent JP2010533483
Kind Code:
A
Abstract:
This invention relates to industrial production of proteins. More specifically, the invention relates to a method for obtaining cells that stably express a protein of interest, even when cultivated in the absence of selective pressure. DHFR is used as a surrogate marker. The transfected cells are not selected based on resistance to a toxic compound, but based on fluorescence as measured by FACS using fluorescent MTX.
Inventors:
Crawford McFadd, Tara Kristen
Yang, Major
Yang, Major
Application Number:
JP2010516504A
Publication Date:
October 28, 2010
Filing Date:
July 16, 2008
Export Citation:
Assignee:
Merck Serono Society Anonym
International Classes:
C12N5/10; C12P21/02
Attorney, Agent or Firm:
Atsushi Aoki
Takashi Ishida
Fukumoto product
Tetsuji Koga
Yoichi Watanabe
Ryotaro Takei
Masaru Nakajima
Takashi Ishida
Fukumoto product
Tetsuji Koga
Yoichi Watanabe
Ryotaro Takei
Masaru Nakajima