Title:
ペグ化ポリペプチドの精製
Document Type and Number:
Japanese Patent JP2010533664
Kind Code:
A
Abstract:
The invention is a method for the purification of mono-PEGylated erythropoietin using two cation exchange chromatography steps wherein the same type of cation exchange material is used in both cation exchange chromatography steps and a method for producing a mono-PEGylated erythropoietin in substantially homogeneous form.
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Inventors:
Burg, Josef
Reichert, Claus
Sucrose, accelerator
Shurig, Hartmut
Wessner, Axel
Reichert, Claus
Sucrose, accelerator
Shurig, Hartmut
Wessner, Axel
Application Number:
JP2010516414A
Publication Date:
October 28, 2010
Filing Date:
July 15, 2008
Export Citation:
Assignee:
F. HOFFMANN-LA ROCHE AKTIENGESELLSCHAFT
International Classes:
C07K14/505; C07K1/18
Domestic Patent References:
| JP2001064300A | 2001-03-13 | |||
| JP2003533487A | 2003-11-11 |
Foreign References:
| WO2009010271A2 | 2009-01-22 | |||
| WO2007039436A1 | 2007-04-12 | |||
| WO2009010271A2 | 2009-01-22 |
Other References:
JPN6012031895; 吉川大和、宮崎香: '「５ イオン交換クロマトグラフィー」' タンパク質実験ノート 下巻, 19960910, Ｐ．１０７-１１４, 株式会社羊土社
Attorney, Agent or Firm:
Hajime Tsukuni
Koshiro Tsukuda
Fusayuki Saito
Koshiro Tsukuda
Fusayuki Saito