Title:
特にリアルタイムPCRによる、核酸の集団の測定
Document Type and Number:
Japanese Patent JP2011502472
Kind Code:
A
Abstract:
The invention relates to a method for measuring the amount of nucleic acids of a target sequence in a sample of interest. The method comprises several steps, and in particular subjecting the sample of interest to an amplification treatment, in the presence of at least one nucleic acids label specific for said target sequence; measuring a physical quantity representative of the evolution of the label; calculating a parameter F0 representative of the physical quantity of the nucleic acids label before any amplification cycle; estimating the initial population size of nucleic acids of the target sequence using a conversion law, comprising contextual parameters, and applied to said parameter F0. The contextual parameters are pre-recorded reference parameters which are substantially independent of at least a part of the experimental conditions. The invention is also directed towards an apparatus comprising units organized for implementing a method for measuring the amount of nucleic acids of a target sequence in a sample of interest.
Inventors:
Jaan virginie
Kaminsky Karine
Kaminsky Karine
Application Number:
JP2010530509A
Publication Date:
January 27, 2011
Filing Date:
October 23, 2008
Export Citation:
Assignee:
Bio-Rad Pasteur
International Classes:
C12Q1/68; C12M1/00; C12N15/09; G06F19/00
Foreign References:
| WO2006048337A1 | 2006-05-11 | |||
| WO2006048337A1 | 2006-05-11 |
Attorney, Agent or Firm:
Koichi Tsujii
Sadao Kumakura
Nobuo Ogawa
Atsushi Hakoda
Kenji Asai
Kazuo Yamazaki
Koji Watanabe
Sadao Kumakura
Nobuo Ogawa
Atsushi Hakoda
Kenji Asai
Kazuo Yamazaki
Koji Watanabe