Title:
改善されたアッセイ
Document Type and Number:
Japanese Patent JP2011505858
Kind Code:
A
Abstract:
The invention relates to process for in vitro prediction of a potentially allergenic substance wherein monocytes and/or macrophages and/or myelomonocytic cell lines are cultivated in the presence of the substance and interferon-γ, whereby productions of cytokines and/or neopterin are increased and measured. The allergic reaction may be estimated by measuring up regulated or down-regulated genes chosen from G1P2, OASL, IFIT1, TRIM22, IFI44L, MXI, RSAD2, IFIT3, IFITM1, IFIT2, C 33.28 HERV-H protein mRNA, IFITM3, XK, GPR15, MT1G, MT1B; MT1A, ADFP, IL8, MT1E, MT1F, MT1H, SLC30A1, SERPINB2, CD83, TncRNA or expression products from them are measured. The invention also relates to a reagent kit for use in the process comprising interferon-γ and reagents which recognise cytokines preferably each of IL-8 and neopterin and/or reagents such as probes recognising up-regulated or down-regulated genes.
Inventors:
Mattson, Karin
Domeika, Christina
Domeika, Christina
Application Number:
JP2010538735A
Publication Date:
March 03, 2011
Filing Date:
December 18, 2008
Export Citation:
Assignee:
Bioveter Technologies Activolag
International Classes:
C12Q1/02; C12Q1/68; G01N33/68; G01N33/53
Attorney, Agent or Firm:
Shinjiro Ono
Kazuo Shamoto
Yasushi Kobayashi
Akio Chiba
Hiroyuki Tomita
Akiko Nakahama
Kazuo Shamoto
Yasushi Kobayashi
Akio Chiba
Hiroyuki Tomita
Akiko Nakahama