Title:
植物プロトプラストへの変異原性核酸塩基のポリエチレングリコール介在導入を用いる改良された突然変異誘発法
Document Type and Number:
Japanese Patent JP2011507505
Kind Code:
A
Abstract:
Method for targeted alteration of a duplex acceptor DNA sequence in a plant cell protoplast, comprising combining the duplex acceptor DNA sequence with a donor mutagenic nucleobase, wherein the duplex acceptor DNA sequence contains a first DNA sequence and a second DNA sequence which is the complement of the first DNA sequence and wherein the donor mutagenic nucleobase comprises at least one mismatch with respect to the duplex acceptor DNA sequence to be altered, preferably with respect to the first DNA sequence, wherein the method further comprises a step of introducing the donor mutagenic nucleobase into the cell protoplasts using polyethylene glycol (PEG) mediated transformation and the use of PEG protoplast transformation for enhancing the rate of targeted mutagenesis.
Inventors:
Bunduk, Paul
Debose, Miheel Theodor Yang
Frank Louis
Debose, Miheel Theodor Yang
Frank Louis
Application Number:
JP2010539319A
Publication Date:
March 10, 2011
Filing Date:
December 21, 2007
Export Citation:
Assignee:
Key Jean NAMROSE FENNOTE SHAP
International Classes:
C12N15/09; C12N15/11; C12N15/82; C12N15/87
Foreign References:
| WO2007073166A1 | 2007-06-28 | |||
| WO2007073170A1 | 2007-06-28 |
Other References:
JPN6012064588; Ｐｌａｎｔａ Ｖｏｌ．２２０, 2005, ｐ．８６４-８７４
JPN6012064591; Ｐｌａｎｔ Ｃｅｌｌ Ｒｅｐ Ｖｏｌ．２５, 2006, ｐ．４５７-４６５
JPN6012064596; Ｃｈｅｍ Ｂｉｏｌ Ｖｏｌ．９, 2002, ｐ．１０７３-１０８４
JPN6012064591; Ｐｌａｎｔ Ｃｅｌｌ Ｒｅｐ Ｖｏｌ．２５, 2006, ｐ．４５７-４６５
JPN6012064596; Ｃｈｅｍ Ｂｉｏｌ Ｖｏｌ．９, 2002, ｐ．１０７３-１０８４
Attorney, Agent or Firm:
Kenho Ikeda
Shuichi Fukuda
Shuichi Fukuda